本研究目的在於評估以超臨界流體(Super critical Fluid, SCF)抽提中藥材蛇床子果實中的蛇床子素(Osthole)，評估各種超臨界抽提變因，並予以最佳化。本研究以二氧化碳作為超臨界流體，評估五種超臨界流體變因分別是夾帶劑、抽提次數、壓力、靜態抽提時間與溫度。以高效率液相層析儀測定抽提效率，固定相為RP-C18管柱，移動相為甲醇:水=7:3，發光二列陣檢測器，最大吸光波長323 nm。最佳化條件為：抽取5公克蛇床子藥材時加甲醇5 mL為夾帶劑，萃取管溫度為攝氏100 ℃，抽提壓力設為0.5 Mpa，靜態抽提時間120分鐘，動態抽提時間20分鐘，反覆以超臨界流體抽提5次；蛇床子素奈米膠漿製備，以卡波姆940的膠體0.25%的濃度，含有蛇床子素0.04%，可使奈米化的蛇床子素維持在奈米等級粒徑的時間較長且不易析出。
This study’s purpose is to estimate using supercritical fluid for extracting Osthole from Cnidium monnieri, compare all those extraction factors and to optimize extraction factors. Use carbon dioxide as a supercritical fluid and estimate five extraction factors as co-solvent, number of extraction, pressure, stastic extraction time and temperature. Used high performance liquid chromatography to detect extraction effect, stastic phase is RP-C18 column and mobile phase MeOH: water=7:3, photo diode array detector set by max absorption wavelength 323 nm. Optimize Result: 5 g herb adds 5 mL MeOH as co-solvent, extraction cube temperature 100℃, extraction pressure 0.5 MPa, extraction time 2 hrs, repeat for 5 times of extraction. Preparation of Osthole nanoemulsion is using 0.25% Carbomer 940 and contain 0.04% Osthole, this condition can maintain nanofabrication’s Osthole in nano level’s particle size for longer time and not easy to dissolve out.
