豬霍亂沙門氏菌 (Salmonella Choleraesuis)是豬常見之致病菌，此感染症在人類也會造成嚴重的系統性感染。過去在臨床上使用抗生素治療此疾病有相當好的效果。然而，也由於抗生素的使用，近年來多重抗藥性的S. Choleraesuis感染也越來越常見。為了瞭解豬和人之間交互感染S. Choleraesuis之相關性，並找出其中可能持續循環的特殊基因型，本研究利用臨床上採集於1997至2002年間54株人之分離株和158株豬之分離株，使用Xba I進行限制酶切割其染色體DNA，再以脈衝式電場電泳 (pulsed-field gel electrophoresis, PFGE)方法進行基因分型分析。結果於豬和人分離株各別找出五種與十一種基因型別。而且在55%豬分離株中之PFGE分型結果，具有高度關性，此叢集型別定義為基因型1型 (gt 1)。與此gt 1相似的基因型同樣可在69%的人類分離株中發現。抗藥性測試結果顯示，豬分離株與人類分離株中分別有96%與88%具有5種或5種以上抗生素之抗藥性。進一步由各年之分布結果來看，gt 1型仍為人類及動物分離株最常見的基因型別。此研究結果充分顯示，相同基因型且具有多重抗藥性的S. Choleraesuis持續在台灣的動物和人之間傳遞。; Salmonella Choleraesuis is a common pathogen in swine population, which also causes serious systematic infection in humans. Antimicrobial treatment was useful in clinical management. However, in recent years, multi-drug-resistance S. Choleraesuis is frequently seen in clinical settings. In order to trace the relationship and find the circulating S. Choleraesuis stains between pigs and humans, chromosomal DNA from 158 pig isolates and 54 human isolates were subjected to molecular analysis by pulsed-field gel electrophoresis (PFGE) with XbaI digestion. In this study, we successfully identified five and nine PFGE genotypes in swine and human isolates, respectively. In the swine isolates, 55% of them were with closely related PFGE patterns, designated as genotype 1 (gt 1). The similar genotype was also identified in 69% of the human isolates. By antimicrobial susceptibility test, 96% and 88% of the swine and human S. Choleraesuis isolates were multi-drug-resistance strains, which were resistant to at least 5 different antibiotics tested. Further stratified by different collection years, S. Choleaesuis with gt1 type was still the most prevalent one in pigs and humans. These observations strongly suggested that multi-drug resistance S. Choleraesuis with unique genotype be circulating between pigs and humans in Taiwan.
