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    Please use this identifier to cite or link to this item: http://ir.cmu.edu.tw/ir/handle/310903500/24314


    Title: 1997-2002人類及豬隻豬霍亂沙門氏菌分離株之分子流行病學及抗藥性趨勢變化研究;Molecular Epidemiology and Antibiotic-resistance Trend Analysis of Salmonella Choleraesuis Isolated from Humans and Pigs in 1997-2002
    Authors: 林怡璇;Lin, Yi-Hsuan
    Contributors: 中國醫藥學院環境醫學研究所
    Keywords: 豬霍亂沙門氏菌;血清鑑定;脈衝式電場電泳;抗生素敏感性試驗;Salmonella enterica subspecies enterica serovar Choleraesuis;serotyping;pulsed-field gel electrophoresis;PFGE;antibiotics test
    Date: 1992
    Issue Date: 2009-12-22 13:57:42 (UTC+8)
    Abstract: 豬霍亂沙門氏菌 (Salmonella Choleraesuis)是豬常見之致病菌，此感染症在人類也會造成嚴重的系統性感染。過去在臨床上使用抗生素治療此疾病有相當好的效果。然而，也由於抗生素的使用，近年來多重抗藥性的S. Choleraesuis感染也越來越常見。為了瞭解豬和人之間交互感染S. Choleraesuis之相關性，並找出其中可能持續循環的特殊基因型，本研究利用臨床上採集於1997至2002年間54株人之分離株和158株豬之分離株，使用Xba I進行限制酶切割其染色體DNA，再以脈衝式電場電泳 (pulsed-field gel electrophoresis, PFGE)方法進行基因分型分析。結果於豬和人分離株各別找出五種與十一種基因型別。而且在55%豬分離株中之PFGE分型結果，具有高度關性，此叢集型別定義為基因型1型 (gt 1)。與此gt 1相似的基因型同樣可在69%的人類分離株中發現。抗藥性測試結果顯示，豬分離株與人類分離株中分別有96%與88%具有5種或5種以上抗生素之抗藥性。進一步由各年之分布結果來看，gt 1型仍為人類及動物分離株最常見的基因型別。此研究結果充分顯示，相同基因型且具有多重抗藥性的S. Choleraesuis持續在台灣的動物和人之間傳遞。; Salmonella Choleraesuis is a common pathogen in swine population, which also causes serious systematic infection in humans. Antimicrobial treatment was useful in clinical management. However, in recent years, multi-drug-resistance S. Choleraesuis is frequently seen in clinical settings. In order to trace the relationship and find the circulating S. Choleraesuis stains between pigs and humans, chromosomal DNA from 158 pig isolates and 54 human isolates were subjected to molecular analysis by pulsed-field gel electrophoresis (PFGE) with XbaI digestion. In this study, we successfully identified five and nine PFGE genotypes in swine and human isolates, respectively. In the swine isolates, 55% of them were with closely related PFGE patterns, designated as genotype 1 (gt 1). The similar genotype was also identified in 69% of the human isolates. By antimicrobial susceptibility test, 96% and 88% of the swine and human S. Choleraesuis isolates were multi-drug-resistance strains, which were resistant to at least 5 different antibiotics tested. Further stratified by different collection years, S. Choleaesuis with gt1 type was still the most prevalent one in pigs and humans. These observations strongly suggested that multi-drug resistance S. Choleraesuis with unique genotype be circulating between pigs and humans in Taiwan.
    Appears in Collections:[Graduate Institute of Environmental Medicine] Theses & dissertations

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    圖三.pdf237KbAdobe PDF1125View/Open
    圖二1.pdf311KbAdobe PDF742View/Open
    圖二2.pdf294KbAdobe PDF945View/Open
    圖二3.pdf305KbAdobe PDF803View/Open
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    圖二5.pdf232KbAdobe PDF735View/Open
    圖五.pdf250KbAdobe PDF827View/Open
    圖六-十三.pdf30KbAdobe PDF923View/Open
    圖十七-1.pdf303KbAdobe PDF785View/Open
    圖十七-2.pdf240KbAdobe PDF944View/Open
    圖十五-1.pdf306KbAdobe PDF826View/Open
    圖十五-2.pdf291KbAdobe PDF759View/Open
    圖十五-3.pdf295KbAdobe PDF893View/Open
    圖十五-4.pdf304KbAdobe PDF769View/Open
    圖十五-5.pdf206KbAdobe PDF752View/Open
    圖十八-1.pdf260KbAdobe PDF765View/Open
    圖十八-2.pdf231KbAdobe PDF804View/Open
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    圖十六-2.pdf249KbAdobe PDF889View/Open
    圖十四-1.pdf269KbAdobe PDF831View/Open
    圖十四-2.pdf299KbAdobe PDF754View/Open
    圖十四-3.pdf220KbAdobe PDF817View/Open
    圖四1.pdf288KbAdobe PDF882View/Open
    圖四2.pdf286KbAdobe PDF924View/Open
    圖目錄.pdf21KbAdobe PDF946View/Open
    摘要 abstract.pdf25KbAdobe PDF1414View/Open
    目錄.pdf23KbAdobe PDF894View/Open
    第一章.pdf25KbAdobe PDF895View/Open
    第七章.pdf50KbAdobe PDF1052View/Open
    第三章.pdf38KbAdobe PDF2603View/Open
    第二章.pdf59KbAdobe PDF7987View/Open
    第五章.pdf29KbAdobe PDF1431View/Open
    第六章.pdf20KbAdobe PDF793View/Open
    第四章.pdf32KbAdobe PDF1100View/Open
    表一.pdf20KbAdobe PDF1110View/Open
    表三.pdf20KbAdobe PDF907View/Open
    表九.十.十一.pdf27KbAdobe PDF1073View/Open
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    表五.pdf21KbAdobe PDF4060View/Open
    表八.pdf21KbAdobe PDF1361View/Open
    表六表七.pdf23KbAdobe PDF877View/Open
    表十二.pdf20KbAdobe PDF828View/Open
    表四.pdf20KbAdobe PDF812View/Open
    表目錄.pdf21KbAdobe PDF1066View/Open
    誌謝.pdf19KbAdobe PDF1192View/Open
    論文封面.pdf20KbAdobe PDF1033View/Open
    附錄一.pdf28KbAdobe PDF1403View/Open
    附錄二.pdf28KbAdobe PDF898View/Open


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