Inactivated macrophage, large amounts of nitric oxide (NO) are generated by inducible nitric oxide synthase (iNOS). This is an important mechanism in macrophage-induced cytotoxicity and inflammation. In this study, we examined the inhibitory effects of a synthetic compound CYL-418b, a derivative of 9-phenoxyacridine, on LPS induced inflammation. CYL-418b can effectively block lipopolysaccharide (LPS) induced NO production in murine macrophage (Raw 264.7) and microglial cell (BV-2) without obvious cytotoxcity. By focusing on the signaling pathway of NO production, CYL-418b did not reduce enzyme activity of iNOS. It inhibited LPS-induced iNOS expression at transcriptional steps. Our data indicated down regulation of NF-κB activity contributed to this inhibitory effect. Effects of CYL-418b on other pro-inflammatory cytokines production in Raw 264.7 and BV-2 stimulated by LPS will be further investigated.
關聯:
The 21th Joint Annual Conference of Biomedical Sciences
