| 摘要: | Natural Product Chemistry
Original Paper Planta Med 2009; 75(5): 512-516
DOI: 10.1055/s-0029-1185305
© Georg Thieme Verlag KG Stuttgart · New York
New Constituents with iNOS Inhibitory Activity from Mycelium of Antrodia camphorata
Sien-Sing Yang1, Guei-Jane Wang2, Shiang-Yi Wang3, Yu-Ying Lin4, Yueh-Hsiung Kuo5, Tzong-Huei Lee4
1 Department of Internal Medicine, Cathy General Hospital, Taipei, Taiwan
2 National Research Institute of Chinese Medicine, Taipei, Taiwan
3 School of Pharmacy, Taipei Medical University, Taipei, Taiwan
4 Graduate Institute of Pharmacognosy, Taipei Medical University, Taipei, Taiwan
5 Tsuzuki Institute for Traditional Medicine, College of Pharmacy, China Medical University, Taichung, Taiwan
Abstract
In continuing our investigation on the bioactive constituents of mycelium of Antrodia camphorata, antroquinonol B (1), 4-acetyl-antroquinonol B (2), 2,3-(methylenedioxy)-6-methylbenzene-1,4-diol (3) and 2,4-dimethoxy-6-methylbenzene-1,3-diol (4) along with antrodin D (5) were isolated by the guidance of an inducible nitric oxide synthase (iNOS) inhibitory assay and identified on the basis of their spectroscopic analysis. The effect of these compounds on the inhibition of NO production in lipopolysaccharide (LPS)-activated murine macrophages was further evaluated. Compounds 4 and 5 significantly inhibited NO production without any cytotoxicity, the IC50 values being 32.2 ± 0.1 and 26.3 ± 1.6 µg/mL, respectively. Compounds 1 and 2 possessed greater effects on NO inhibition, with IC50 values of 16.2 ± 0.8 and 14.7 ± 2.8 µg/mL, respectively, but displayed cytotoxicity at considerably higher concentrations. Compound 3 showed the lowest percent cell viability of 45.5 ± 1.8 % as observed in treated cells at a concentration of 16.8 µg/mL. |
