New constituents with iNOS inhibitory activity from mycelium of Antrodia camphorata

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Title:	New constituents with iNOS inhibitory activity from mycelium of Antrodia camphorata
Authors:	(Sien-Sing Yang);(Guei-Jane Wang);(Shiang-Yi Wang);(Yu-Ying Lin);郭悅雄;(Tzong-Huei Lee)*
Contributors:	藥學院中國藥學研究所;中國附醫院長室
Keywords:	Antrodia camphorata;Polyporaceae;mycelium;antroquinonol B;iNOS
Date:	2009
Issue Date:	2009-08-26 16:20:01 (UTC+8)
Abstract:	Natural Product Chemistry Original Paper Planta Med 2009; 75(5): 512-516 DOI: 10.1055/s-0029-1185305 © Georg Thieme Verlag KG Stuttgart · New York New Constituents with iNOS Inhibitory Activity from Mycelium of Antrodia camphorata Sien-Sing Yang1, Guei-Jane Wang2, Shiang-Yi Wang3, Yu-Ying Lin4, Yueh-Hsiung Kuo5, Tzong-Huei Lee4 1 Department of Internal Medicine, Cathy General Hospital, Taipei, Taiwan 2 National Research Institute of Chinese Medicine, Taipei, Taiwan 3 School of Pharmacy, Taipei Medical University, Taipei, Taiwan 4 Graduate Institute of Pharmacognosy, Taipei Medical University, Taipei, Taiwan 5 Tsuzuki Institute for Traditional Medicine, College of Pharmacy, China Medical University, Taichung, Taiwan Abstract In continuing our investigation on the bioactive constituents of mycelium of Antrodia camphorata, antroquinonol B (1), 4-acetyl-antroquinonol B (2), 2,3-(methylenedioxy)-6-methylbenzene-1,4-diol (3) and 2,4-dimethoxy-6-methylbenzene-1,3-diol (4) along with antrodin D (5) were isolated by the guidance of an inducible nitric oxide synthase (iNOS) inhibitory assay and identified on the basis of their spectroscopic analysis. The effect of these compounds on the inhibition of NO production in lipopolysaccharide (LPS)-activated murine macrophages was further evaluated. Compounds 4 and 5 significantly inhibited NO production without any cytotoxicity, the IC50 values being 32.2 ± 0.1 and 26.3 ± 1.6 µg/mL, respectively. Compounds 1 and 2 possessed greater effects on NO inhibition, with IC50 values of 16.2 ± 0.8 and 14.7 ± 2.8 µg/mL, respectively, but displayed cytotoxicity at considerably higher concentrations. Compound 3 showed the lowest percent cell viability of 45.5 ± 1.8 % as observed in treated cells at a concentration of 16.8 µg/mL.
Relation:	PLANTA MEDICA 75(75)512~516
Appears in Collections:	[Graduate Institute of Chinese Pharmaceutical Science] Journal articles

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