Molecular analysis of the thiopurine S-methyltransferase alleles in Bolivians and Tibetans

中國醫藥大學機構典藏 China Medical University Repository, Taiwan > 醫學院 > 醫學研究所 > 期刊論文 > Item 310903500/7231

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题名:	Molecular analysis of the thiopurine S-methyltransferase alleles in Bolivians and Tibetans
作者:	呂旭峰(Lu H.F.);施木青(Shih M.C.);薛樹清(Hsueh S.C.);陳志玫(Chen C.M.);張建裕(Chang J.Y.);張建國(Chang J.G.)*
贡献者:	醫學院醫學研究所;中國附醫檢驗醫學部
日期:	2005-10
上传时间:	2009-08-26 16:16:38 (UTC+8)
摘要:	BACKGROUND: Thiopurine drugs are used as immunosuppressant or cytotoxic drugs. Thiopurine S-methyltransferase (TPMT) methylates and thereby modulates the therapeutic and toxic effects of these drugs. The activity of TPMT is affected by genetic polymorphism of TPMT alleles, and these alleles have not been studied in Tibetans and Bolivians. OBJECTIVES: To analyse the TPMT allelic frequencies in Tibetans and Bolivians. METHODS: We developed an inexpensive method for collecting blood and extracting genomic DNA. Genomic DNA was extracted from blood spots of 50 Tibetans and 115 Bolivians. The frequencies of allelic variants of TPMT gene (TPMT1 to TPMT8) were determined using polymerase chain reaction-restriction fragment length polymorphism technique. RESULTS: The allelic frequencies of TPMT1 were 99 and 93.48% for Tibetans and Bolivians, respectively. The corresponding allelic frequencies of TPMT3A were 0 and 6.52% and those of TPMT3C were 1.0 and 0%. No TPMT2, 3B, 3D, 4-8 were found in these two populations. CONCLUSIONS: As with Caucasian populations, TPMT*3A is the most prevalent mutant allele in Bolivians. Our results may be of value in helping to guide the prescription of thiopurine drugs in these populations.
關聯:	JOURNAL OF CLINICAL PHARMACY AND THERAPEUTICS 30(5 )491 ~496
显示于类别:	[醫學研究所] 期刊論文

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