Molecular analysis of the thiopurine S-methyltransferase alleles in Bolivians and Tibetans

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Title:	Molecular analysis of the thiopurine S-methyltransferase alleles in Bolivians and Tibetans
Authors:	呂旭峰(Lu H.F.);施木青(Shih M.C.);薛樹清(Hsueh S.C.);陳志玫(Chen C.M.);張建裕(Chang J.Y.);張建國(Chang J.G.)*
Contributors:	醫學院醫學研究所;中國附醫檢驗醫學部
Date:	2005-10
Issue Date:	2009-08-26 16:16:38 (UTC+8)
Abstract:	BACKGROUND: Thiopurine drugs are used as immunosuppressant or cytotoxic drugs. Thiopurine S-methyltransferase (TPMT) methylates and thereby modulates the therapeutic and toxic effects of these drugs. The activity of TPMT is affected by genetic polymorphism of TPMT alleles, and these alleles have not been studied in Tibetans and Bolivians. OBJECTIVES: To analyse the TPMT allelic frequencies in Tibetans and Bolivians. METHODS: We developed an inexpensive method for collecting blood and extracting genomic DNA. Genomic DNA was extracted from blood spots of 50 Tibetans and 115 Bolivians. The frequencies of allelic variants of TPMT gene (TPMT1 to TPMT8) were determined using polymerase chain reaction-restriction fragment length polymorphism technique. RESULTS: The allelic frequencies of TPMT1 were 99 and 93.48% for Tibetans and Bolivians, respectively. The corresponding allelic frequencies of TPMT3A were 0 and 6.52% and those of TPMT3C were 1.0 and 0%. No TPMT2, 3B, 3D, 4-8 were found in these two populations. CONCLUSIONS: As with Caucasian populations, TPMT*3A is the most prevalent mutant allele in Bolivians. Our results may be of value in helping to guide the prescription of thiopurine drugs in these populations.
Relation:	JOURNAL OF CLINICAL PHARMACY AND THERAPEUTICS 30(5 )491 ~496
Appears in Collections:	[Graduate Institute of Medical Science] Journal articles

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