Studies on the Chemical Constituents of Crysinus hastatus（Thunb.）Copel.
Yi-ru Chen
Institute of Chinese Pharmaceutical Sciences
China Medical University
Abstract
Crypsinus hastatus（Thunb.）Copel. is a native plant of Taiwan belonging to Polypodiaceae family. It is a common folk medicine used for dispelling colds, detumescence, tubercle bacillus, hepatitis, dysentery and detoxication, etc. It grows under the rock at mountain areas in Taiwan.
The methanol extract of whole plant C. hastatus was fractionated into n-hexane, chloroform, ethyl acetate, n-butanol and aqueous fractions. The antioxidant activity of methanol extract and various fractions were evaluated by DPPH free radical scavenging activity model. The acute toxicity of methanol extract in rats was also evaluated. The result revealed that the n-butanol fraction at two milligram per milliliter exhibited 95% antioxidant activity was ninty-five percent. It exhibited better antioxidant potency than ethyl acetate and chloroform fractions in DPPH free radical scavenging activity. In the acute toxicity test the LD50 of the methanol extract of C. hastatus was larger than 10g/kg. It is considered relatively safe.
In the phytochemical study, compounds isolated from Crypsinus hastatus were identified as coumarin (H-1), β-sitosterol (H-2), 20-hydroxyecdysone (E-1)、β-ecdysone (E-2) and naringin (E-3). The coumarin and naringin contants in the methanol extract of C. hastatus were also analyzed as 0.0803% and 5.6% separately.
