Ergothioneine protects against neuronal injury induced by cisplatin both in vitro and in vivo

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Title:	Ergothioneine protects against neuronal injury induced by cisplatin both in vitro and in vivo
Authors:	宋祖瑩(Tuzz-Ying Song)*;(Chien-Lin Chen);(Jiunn-Wang Liao);歐秀中(Hsiu-Chung Ou);(Ming-Shiun Tsai)
Contributors:	物理治療學系
Keywords:	Ergothioneine;Cisplatin;Avoidance tests;Neuron cells;Acetylcholinesterase activity;Oxidative stress
Date:	2010-12
Issue Date:	2010-11-19 16:54:53 (UTC+8)
Abstract:	The neuroprotective effects of ergothioneine (EGT) against cisplatin toxicity were investigated both in vitro and in vivo. For in vitro study, two types of neuronal cells, primary cortical neuron (PCN) cells and rat pheochromocytoma (PC12) cells, were incubated with EGT (0.1–10.0 lM) for 2 h followed by incubation with 0.5 lM cisplatin for 72 h. Results show that cisplatin markedly decreased the proliferation of PC12 cells and strongly inhibited the growth of axon and dendrite of PCN cells, but these effects were significantly prevented by EGT. For in vivo study, CBA mice were orally administered with 2 or 8 mg EGT/kg body weight for 58 consecutive days and were injected i.p. with 5 mg cisplatin/kg body weight on days 7, 9 and 11. We found that EGT significantly restored the learning and memory deficits in mice treated with cisplatin evaluated by active and passive avoidance tests. EGT also significantly prevented brain lipid peroxidation, restored acetylcholinesterase (AChE) activity and maintained glutathione/glutathione disulfide ratio in brain tissues of mice treated with cisplatin. These results demonstrate that EGT protects against cisplatin-induced neuronal injury and enhances cognition, possibly through the inhibition of oxidative stress and restoration of AChE activity in neuronal cells.
Relation:	FOOD AND CHEMICAL TOXICOLOGY 48(12):3492-3499
Appears in Collections:	[Department of Physical Therapy, Graduate Institute of Rehabilitation Science] Journal articles

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