中國醫藥大學機構典藏 China Medical University Repository, Taiwan:Item 310903500/3274
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    請使用永久網址來引用或連結此文件: http://ir.cmu.edu.tw/ir/handle/310903500/3274


    題名: Comparison of pulse-field gel electrophoresis and three rep-PCR methods for evaluating the genetic relatedness of Stenotrophomonas maltophilia isolates
    作者: 林振文(Lin Cheng Wen);邱乾順(Chien-Shun Chiou);張益銍(Yi-Chih, Chang);楊翠青(Tsuey-Ching Yang)*
    貢獻者: 健康照護學院醫學檢驗生物技術學系;中國附醫檢驗醫學部
    關鍵詞: BOX-PCR;enterobacterial repetitive intergenic consensus-PCR;pulsed-field gel electrophoresis;repetitive extragenic palindromic-PCR;Stenotrophomonas maltophilia
    日期: 2008-10
    上傳時間: 2009-08-20 18:29:04 (UTC+8)
    摘要: Aims: In this study, three facile repetitive-sequence PCR (rep-PCR) techniques have been compared with the pulsed-field gel electrophoresis (PFGE) method for differentiating the genetic relatedness of clinical Stenotrophomonas maltophilia isolates.

    Methods and Results: The dendrograms of 20 S. maltophilia isolates were constructed based on the data obtained from PFGE and three PCR-based methods, i.e. enterobacterial repetitive intergenic consensus-PCR (ERIC-PCR), BOX-PCR and repetitive extragenic palindromic-PCR (REP-PCR). When compared with PFGE, ERIC-PCR displayed a much lower discriminatory power, whereas BOX-PCR and REP-PCR had a comparable discriminatory power for close genetic-related isolates.

    Conclusion: BOX-PCR and REP-PCR can be convenient and effective methods for evaluating the close genetic relatedness of clinical S. maltophilia isolates.

    Significance and Impact of the Study: A rapid method for determining S. maltophilia's close genetic relatedness provides a convenient tool for understanding the epidemiology of S. maltophilia.
    關聯: LETTERS IN APPLIED MICROBIOLOGY 47(5):393~398
    顯示於類別:[醫學檢驗生物技術學系暨碩士班 ] 期刊論文

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