Comparison of pulse-field gel electrophoresis and three rep-PCR methods for evaluating the genetic relatedness of Stenotrophomonas maltophilia isolates

中國醫藥大學機構典藏 China Medical University Repository, Taiwan > 醫學院 > 醫學檢驗生物技術學系暨碩士班 > 期刊論文 > Item 310903500/3274

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题名:	Comparison of pulse-field gel electrophoresis and three rep-PCR methods for evaluating the genetic relatedness of Stenotrophomonas maltophilia isolates
作者:	林振文(Lin Cheng Wen);邱乾順(Chien-Shun Chiou);張益銍(Yi-Chih, Chang);楊翠青(Tsuey-Ching Yang)*
贡献者:	健康照護學院醫學檢驗生物技術學系;中國附醫檢驗醫學部
关键词:	BOX-PCR;enterobacterial repetitive intergenic consensus-PCR;pulsed-field gel electrophoresis;repetitive extragenic palindromic-PCR;Stenotrophomonas maltophilia
日期:	2008-10
上传时间:	2009-08-20 18:29:04 (UTC+8)
摘要:	Aims: In this study, three facile repetitive-sequence PCR (rep-PCR) techniques have been compared with the pulsed-field gel electrophoresis (PFGE) method for differentiating the genetic relatedness of clinical Stenotrophomonas maltophilia isolates. Methods and Results: The dendrograms of 20 S. maltophilia isolates were constructed based on the data obtained from PFGE and three PCR-based methods, i.e. enterobacterial repetitive intergenic consensus-PCR (ERIC-PCR), BOX-PCR and repetitive extragenic palindromic-PCR (REP-PCR). When compared with PFGE, ERIC-PCR displayed a much lower discriminatory power, whereas BOX-PCR and REP-PCR had a comparable discriminatory power for close genetic-related isolates. Conclusion: BOX-PCR and REP-PCR can be convenient and effective methods for evaluating the close genetic relatedness of clinical S. maltophilia isolates. Significance and Impact of the Study: A rapid method for determining S. maltophilia's close genetic relatedness provides a convenient tool for understanding the epidemiology of S. maltophilia.
關聯:	LETTERS IN APPLIED MICROBIOLOGY 47(5):393~398
显示于类别:	[醫學檢驗生物技術學系暨碩士班 ] 期刊論文

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