CPAP is a cell-cycle regulated protein that controls centriole length

CMUR > College of Medicine > Graduate Institute of Immunology > Journal articles > Item 310903500/31147

Please use this identifier to cite or link to this item: http://ir.cmu.edu.tw/ir/handle/310903500/31147

Title:	CPAP is a cell-cycle regulated protein that controls centriole length
Authors:	唐張潔如(Chieh-Ju C. Tang);傅如輝(Ru-Huei Fu);吳國盛(Kuo-Sheng Wu);許文彬(Wen-Bin Hsu);唐堂(Tang K. Tang)*
Contributors:	醫學院免疫學研究所;中國附醫神經精神醫學中心
Date:	2009-07
Issue Date:	2010-09-27 15:43:02 (UTC+8)
Abstract:	Centriole duplication involves the growing of a procentriole (progeny centriole) next to the proximal end of each pre-existing centriole (parental centriole). The molecular mechanisms that regulate procentriole elongation remain obscure. We show here that expression of the centriolar protein CPAP (centrosomal P4.1-associated protein) is carefully regulated during the cell cycle, with the protein being degraded in late mitosis. Depletion of CPAP inhibited centrosome duplication, whereas excess CPAP induced the formation of elongated procentriole-like structures (PLSs), which contain stable microtubules and several centriolar proteins. Ultrastructural analysis revealed that these structures are similar to procentrioles with elongated microtubules. Overexpression of a CPAP mutant (CPAP-377EE) that does not bind to tubulin dimers significantly inhibited the formation of CPAP-induced PLSs. Together, these results suggest that CPAP is a new regulator of centriole length and its intrinsic tubulin-dimer binding activity is required for procentriole elongation.
Relation:	NATURE CELL BIOLOGY 11(7):825-831
Appears in Collections:	[Graduate Institute of Immunology] Journal articles

Files in This Item:

File	Description	Size	Format
index.html		0Kb	HTML	621	View/Open

Loading...