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資料載入中.....
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請使用永久網址來引用或連結此文件:
http://ir.cmu.edu.tw/ir/handle/310903500/31043
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| 題名: | 2-Phenyl-4-quinolone prevents serotonin-induced increases in endothelial permeability to albumin |
| 作者: | Lee, HZ;Lin, WC;Yeh, FT;Wu, CH |
| 貢獻者: | 藥學院藥學系;China Med Coll, Sch Pharm, Taichung 404, Taiwan;China Med Coll, Grad Inst Chinese Pharmaceut Sci, Taichung 404, Taiwan;Acad Sinica, Inst Biomed Sci, Taipei, Taiwan |
| 日期: | 1998 |
| 上傳時間: | 2010-09-24 18:55:13 (UTC+8) |
| 出版者: | ELSEVIER SCIENCE BV |
| 摘要: | It is known, from the zymogram method of nuclease activity assay, that the crude extracts of CI cunninghamella echinulata var. echinulata contained at least three distinct extracellular nucleases. Among them, the major form was 30 kDa in molecular mass and termed nuclease C1. In this report, nuclease C1 was purified to apparent homogeneity by chromatography on Cibacron blue-3GA, phenyl-Sepharose 4B and HiTrap Heparin. Nuclease C1 acquired enzymatic activity in the presence of Mn2+ or Mg2+ and was inhibited by EDTA. The activity was maximal at pH 7-8.5. The primary structure of nuclease C1 was completely determined using enzymatic digestion and gene cloning. The N-terminal 49 residues of nuclease C1 were first elucidated from a tryptic digest. Two degenerate upstream primers were subsequently designed to amplify the cDNA encoding nuclease C1. The resulting protein sequence of nuclease C1 was shown to be composed of 252 residues. It was intriguing to find that the protein sequence of nuclease C1 showed significant similarities with the sequences of the mitochondrial nucleases of Saccharomyces cervisiae (44% to identity) and Schizosaccharomyces pombe (42% identity). Residue His87 of nuclease C1 was postulated to he located at the active site from sequence similarity with secreted nuclease from Serratia,marcescens. |
| 關聯: | EUROPEAN JOURNAL OF PHARMACOLOGY 354(2月3日):205-213 |
| 顯示於類別: | [藥學系暨碩博士班] 期刊論文
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