2-Phenyl-4-quinolone prevents serotonin-induced increases in endothelial permeability to albumin

CMUR > College of Pharmacy > School of Pharmacy/Master Degree Program, Ph. D program > Journal articles > Item 310903500/31043

Please use this identifier to cite or link to this item: http://ir.cmu.edu.tw/ir/handle/310903500/31043

Title:	2-Phenyl-4-quinolone prevents serotonin-induced increases in endothelial permeability to albumin
Authors:	Lee, HZ;Lin, WC;Yeh, FT;Wu, CH
Contributors:	藥學院藥學系;China Med Coll, Sch Pharm, Taichung 404, Taiwan;China Med Coll, Grad Inst Chinese Pharmaceut Sci, Taichung 404, Taiwan;Acad Sinica, Inst Biomed Sci, Taipei, Taiwan
Date:	1998
Issue Date:	2010-09-24 18:55:13 (UTC+8)
Publisher:	ELSEVIER SCIENCE BV
Abstract:	It is known, from the zymogram method of nuclease activity assay, that the crude extracts of CI cunninghamella echinulata var. echinulata contained at least three distinct extracellular nucleases. Among them, the major form was 30 kDa in molecular mass and termed nuclease C1. In this report, nuclease C1 was purified to apparent homogeneity by chromatography on Cibacron blue-3GA, phenyl-Sepharose 4B and HiTrap Heparin. Nuclease C1 acquired enzymatic activity in the presence of Mn2+ or Mg2+ and was inhibited by EDTA. The activity was maximal at pH 7-8.5. The primary structure of nuclease C1 was completely determined using enzymatic digestion and gene cloning. The N-terminal 49 residues of nuclease C1 were first elucidated from a tryptic digest. Two degenerate upstream primers were subsequently designed to amplify the cDNA encoding nuclease C1. The resulting protein sequence of nuclease C1 was shown to be composed of 252 residues. It was intriguing to find that the protein sequence of nuclease C1 showed significant similarities with the sequences of the mitochondrial nucleases of Saccharomyces cervisiae (44% to identity) and Schizosaccharomyces pombe (42% identity). Residue His87 of nuclease C1 was postulated to he located at the active site from sequence similarity with secreted nuclease from Serratia,marcescens.
Relation:	EUROPEAN JOURNAL OF PHARMACOLOGY 354(2月3日):205-213
Appears in Collections:	[School of Pharmacy/Master Degree Program, Ph. D program] Journal articles

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