Effects of ibuprofen on arylamine N-acetyltransferase activity in human colon tumor cells

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Title:	Effects of ibuprofen on arylamine N-acetyltransferase activity in human colon tumor cells
Authors:	Chung, JG;Chang, HL;Lin, WC;Yeh, FT;Hung, CF
Contributors:	醫學院醫學系微生物學科;China Med Coll, Dept Microbiol, Taichung 400, Taiwan;China Med Coll, Dept Pharmacol, Taichung 400, Taiwan;China Med Coll, Grad Inst Chinese Pharmaceut Sci, Taichung 400, Taiwan;Taipei Municipal Jen Ai Hosp, Dept Surg, Taichung 400, Taiwan
Date:	1999
Issue Date:	2010-09-24 14:59:45 (UTC+8)
Publisher:	JOHN WILEY & SONS LTD
Abstract:	Carcinoma of the uterine cervix is a common malignancy among women that has been found to show loss of heterozygosity in the chromosome 11p. Recent studies have localized the TSG101 gene in this region, and also demonstrated a high frequency of abnormalities of this gene in human breast cancer. To determine the role of the TSG101 gene in the carcinogenesis of cervical and uterine carcinoma, 19 cases of cervical carcinoma and five cases of endometrial carcinoma,as well as nearby non-cancerous tissue from the same patients, and 16 blood samples from healthy persons as normal control were analysed by Southern blot analysis of genomic DNA, reverse transcription of the TSG101 mRNA followed by PCR amplification and sequencing of the products. We found that abnormal transcripts of the TSG101 gene were common both in cancerous or non-cancerous tissues of the uterus and cervix and in normal peripheral mononuclear cells. There was no genomic deletion or rearrangement in spite of the presence of abnormal transcripts, and no definite relationship between the abnormal transcripts and HPV infection was found. Although the frequency of abnormal transcripts was higher in cancerous than in non-cancerous tissue, normal peripheral mononuclear cells also had abnormal transcripts. Given these findings, the role of the TSG101 gene as a tumour-suppressor gene should be re-evaluated. Because some aberrant transcripts could be found at the first PCR reaction, we suggest that the aberrant transcripts might be the result of imperfect minor splicesome products.
Relation:	JOURNAL OF APPLIED TOXICOLOGY 19(1):1-6
Appears in Collections:	[School of Medicine] Journal articles

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