Effects of ibuprofen on arylamine N-acetyltransferase activity in human colon tumor cells

中國醫藥大學機構典藏 China Medical University Repository, Taiwan > 醫學院 > 醫學系 > 期刊論文 > Item 310903500/30671

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題名:	Effects of ibuprofen on arylamine N-acetyltransferase activity in human colon tumor cells
作者:	Chung, JG;Chang, HL;Lin, WC;Yeh, FT;Hung, CF
貢獻者:	醫學院醫學系微生物學科;China Med Coll, Dept Microbiol, Taichung 400, Taiwan;China Med Coll, Dept Pharmacol, Taichung 400, Taiwan;China Med Coll, Grad Inst Chinese Pharmaceut Sci, Taichung 400, Taiwan;Taipei Municipal Jen Ai Hosp, Dept Surg, Taichung 400, Taiwan
日期:	1999
上傳時間:	2010-09-24 14:59:45 (UTC+8)
出版者:	JOHN WILEY & SONS LTD
摘要:	Carcinoma of the uterine cervix is a common malignancy among women that has been found to show loss of heterozygosity in the chromosome 11p. Recent studies have localized the TSG101 gene in this region, and also demonstrated a high frequency of abnormalities of this gene in human breast cancer. To determine the role of the TSG101 gene in the carcinogenesis of cervical and uterine carcinoma, 19 cases of cervical carcinoma and five cases of endometrial carcinoma,as well as nearby non-cancerous tissue from the same patients, and 16 blood samples from healthy persons as normal control were analysed by Southern blot analysis of genomic DNA, reverse transcription of the TSG101 mRNA followed by PCR amplification and sequencing of the products. We found that abnormal transcripts of the TSG101 gene were common both in cancerous or non-cancerous tissues of the uterus and cervix and in normal peripheral mononuclear cells. There was no genomic deletion or rearrangement in spite of the presence of abnormal transcripts, and no definite relationship between the abnormal transcripts and HPV infection was found. Although the frequency of abnormal transcripts was higher in cancerous than in non-cancerous tissue, normal peripheral mononuclear cells also had abnormal transcripts. Given these findings, the role of the TSG101 gene as a tumour-suppressor gene should be re-evaluated. Because some aberrant transcripts could be found at the first PCR reaction, we suggest that the aberrant transcripts might be the result of imperfect minor splicesome products.
關聯:	JOURNAL OF APPLIED TOXICOLOGY 19(1):1-6
顯示於類別:	[醫學系] 期刊論文

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