Effect of young barley leaf extract and adlay on plasma lipids and LDL oxidation in hyperlipidemic smokers

中國醫藥大學機構典藏 China Medical University Repository, Taiwan > 醫學院 > 醫學系 > 期刊論文 > Item 310903500/30526

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题名:	Effect of young barley leaf extract and adlay on plasma lipids and LDL oxidation in hyperlipidemic smokers
作者:	Yu, YM;Chang, WC;Liu, CS;Tsai, CM
贡献者:	醫學院醫學系家醫學科;Natl Chung Yuan Univ, Dept Biosci Technol, Chungli 320, Taiwan;China Med Univ, Dept Family Med, Taichung 404, Taiwan;China Med Univ, Dept Sport Med, Taichung 404, Taiwan;China Med Univ, Dept Physiol, Taichung 404, Taiwan;China Med Univ, Dept Nutr, Taichung 404, Taiwan
日期:	2004
上传时间:	2010-09-24 14:57:02 (UTC+8)
出版者:	PHARMACEUTICAL SOC JAPAN
摘要:	Purpose: This study determined the incidence of sperm nuclear DNA fragmentation, mitochondrial dysfunction, and chromosomal aneuploidy. The results were correlated with the semen analysis parameters and fertilization rates. Methods: Semen samples from 10 men showing oligoasthenoteratozoospermia (OAT) and undergoing ICSI treatment were analyzed. Another semen samples from 10 men showing normozoospermia and undergoing IVF treatment were analyzed for comparison. The samples were prepared using a two-step discontinuous Percoll gradient (80%-50%) and analyzed using a Hamilton-Thorne Integrated Visual Optical System (IVOS) Sperm Analyzer. DNA fragmentation was detected with a terminal deoxynucleotidyl transferase-mediated dUTP nick end label (TUNEL) assay. Functional integrity of mitochondria was detected using an Apoalert(TM) Mitochondrial Membrane Sensor Kit. Chromosomal aneuploidy was assayed by fluorescence in situ hybridization. Results: Higher sperm DNA fragmentation rate (18.8% vs. 2.8%), mitochondrial dysfunction rate (24.9% vs. 5.7%), and chromosomal aneuploidy rate (0.12% vs. 0.06%) were found in the oligoasthenoteratozoospermic patients in comparison with the normozoospermic patients. Conclusions: The result indicates that spermatozoa from oligoasthenoteratozoospermic patients contain greater DNA fragmentation, mitochondrial dysfunction, and chromosomal aneuploidy. Because extremely poor semen samples are the indication for ICSI treatment, the result indicates the importance of selecting good quality sperm for oocyte injection.
關聯:	BIOLOGICAL & PHARMACEUTICAL BULLETIN 27(6):802-805
显示于类别:	[醫學系] 期刊論文

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