Effect of young barley leaf extract and adlay on plasma lipids and LDL oxidation in hyperlipidemic smokers

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Title:	Effect of young barley leaf extract and adlay on plasma lipids and LDL oxidation in hyperlipidemic smokers
Authors:	Yu, YM;Chang, WC;Liu, CS;Tsai, CM
Contributors:	醫學院醫學系家醫學科;Natl Chung Yuan Univ, Dept Biosci Technol, Chungli 320, Taiwan;China Med Univ, Dept Family Med, Taichung 404, Taiwan;China Med Univ, Dept Sport Med, Taichung 404, Taiwan;China Med Univ, Dept Physiol, Taichung 404, Taiwan;China Med Univ, Dept Nutr, Taichung 404, Taiwan
Date:	2004
Issue Date:	2010-09-24 14:57:02 (UTC+8)
Publisher:	PHARMACEUTICAL SOC JAPAN
Abstract:	Purpose: This study determined the incidence of sperm nuclear DNA fragmentation, mitochondrial dysfunction, and chromosomal aneuploidy. The results were correlated with the semen analysis parameters and fertilization rates. Methods: Semen samples from 10 men showing oligoasthenoteratozoospermia (OAT) and undergoing ICSI treatment were analyzed. Another semen samples from 10 men showing normozoospermia and undergoing IVF treatment were analyzed for comparison. The samples were prepared using a two-step discontinuous Percoll gradient (80%-50%) and analyzed using a Hamilton-Thorne Integrated Visual Optical System (IVOS) Sperm Analyzer. DNA fragmentation was detected with a terminal deoxynucleotidyl transferase-mediated dUTP nick end label (TUNEL) assay. Functional integrity of mitochondria was detected using an Apoalert(TM) Mitochondrial Membrane Sensor Kit. Chromosomal aneuploidy was assayed by fluorescence in situ hybridization. Results: Higher sperm DNA fragmentation rate (18.8% vs. 2.8%), mitochondrial dysfunction rate (24.9% vs. 5.7%), and chromosomal aneuploidy rate (0.12% vs. 0.06%) were found in the oligoasthenoteratozoospermic patients in comparison with the normozoospermic patients. Conclusions: The result indicates that spermatozoa from oligoasthenoteratozoospermic patients contain greater DNA fragmentation, mitochondrial dysfunction, and chromosomal aneuploidy. Because extremely poor semen samples are the indication for ICSI treatment, the result indicates the importance of selecting good quality sperm for oocyte injection.
Relation:	BIOLOGICAL & PHARMACEUTICAL BULLETIN 27(6):802-805
Appears in Collections:	[School of Medicine] Journal articles

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