資料載入中.....
|
請使用永久網址來引用或連結此文件:
http://ir.cmu.edu.tw/ir/handle/310903500/29904
|
| 題名: | Interleukin-1 beta and interleukin-1 receptor antagonist gene polymorphisms in rheumatoid arthritis |
| 作者: | Huang, CM;Tsai, FJ;Wu, JY;Wu, MC |
| 貢獻者: | 附設醫院基因醫學部;China Med Coll Hosp, Dept Med Genet, Taichung, Taiwan;China Med Coll Hosp, Dept Internal Med, Div Rheumatol & Immunol, Taichung, Taiwan |
| 日期: | 2001 |
| 上傳時間: | 2010-09-24 14:45:15 (UTC+8) |
| 出版者: | TAYLOR & FRANCIS AS |
| 摘要: | Tc-99m sestamibi (MIBI) has been used as a tumor-seeking agent. However, its role in detecting lymphomas has not been widely investigated. The aim of the present study was to determine the uptake and clearance characteristics of Tc-99m MIBI in vincristine-resistant lymphoma cell lines. In addition, thallium-201 (TI-201) and gallium-67 (Ga-67) uptake and clearance characteristics were evaluated for comparison with Tc-99m MIBI. Drug-resistant lymphoma cell lines (monocyte-like, histiocytic lymphoma. human; B-lymphoma cell line, American Burkitt lymphoma, lymphoblastoid. human; Hodgkin's disease, lymphoid, human) were selected by multistep vincristine treatment up to 50 nM. After incubation of the radiotracers, Tc-99m MIBI,TI-201 and Ga-67, in medium for 0, 10, 20, 30, 60 or 120 min, the uptake and clearance of each radiotracer were measured in the drug-resistant lymphoma cell lines. In addition, P-glycoprotein expression was determined by immunohistochemical study. In a comparison of the three radiotracers, the uptake of Tc-99m MIBI was the greatest in the studied wild-type lymphoma cell lines. Tc-99m MIBI uptake was much lower in drug-resistant tumor cell lines than in non-resistant cell lines. On the other hand, the uptake characteristics of TI-201 did not differ between drug-resistant and non-resistant cells. Immunohistochemistry analyses of Ab-1 or JSB indicated that tumor cells expressed MDR-1 protein in all three cell lines. Tc-99m MIBI is a good radiotracer for detecting drug resistance in lymphoma cell lines. (C) 2001 Elsevier Science Ireland Ltd. All rights reserved. |
| 關聯: | SCANDINAVIAN JOURNAL OF RHEUMATOLOGY 30(4):225-228 |
| 顯示於類別: | [台中附設醫院] 期刊論文
|
在CMUR中所有的資料項目都受到原著作權保護.
|
