Interleukin-1 beta and interleukin-1 receptor antagonist gene polymorphisms in rheumatoid arthritis

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Title:	Interleukin-1 beta and interleukin-1 receptor antagonist gene polymorphisms in rheumatoid arthritis
Authors:	Huang, CM;Tsai, FJ;Wu, JY;Wu, MC
Contributors:	附設醫院基因醫學部;China Med Coll Hosp, Dept Med Genet, Taichung, Taiwan;China Med Coll Hosp, Dept Internal Med, Div Rheumatol & Immunol, Taichung, Taiwan
Date:	2001
Issue Date:	2010-09-24 14:45:15 (UTC+8)
Publisher:	TAYLOR & FRANCIS AS
Abstract:	Tc-99m sestamibi (MIBI) has been used as a tumor-seeking agent. However, its role in detecting lymphomas has not been widely investigated. The aim of the present study was to determine the uptake and clearance characteristics of Tc-99m MIBI in vincristine-resistant lymphoma cell lines. In addition, thallium-201 (TI-201) and gallium-67 (Ga-67) uptake and clearance characteristics were evaluated for comparison with Tc-99m MIBI. Drug-resistant lymphoma cell lines (monocyte-like, histiocytic lymphoma. human; B-lymphoma cell line, American Burkitt lymphoma, lymphoblastoid. human; Hodgkin's disease, lymphoid, human) were selected by multistep vincristine treatment up to 50 nM. After incubation of the radiotracers, Tc-99m MIBI,TI-201 and Ga-67, in medium for 0, 10, 20, 30, 60 or 120 min, the uptake and clearance of each radiotracer were measured in the drug-resistant lymphoma cell lines. In addition, P-glycoprotein expression was determined by immunohistochemical study. In a comparison of the three radiotracers, the uptake of Tc-99m MIBI was the greatest in the studied wild-type lymphoma cell lines. Tc-99m MIBI uptake was much lower in drug-resistant tumor cell lines than in non-resistant cell lines. On the other hand, the uptake characteristics of TI-201 did not differ between drug-resistant and non-resistant cells. Immunohistochemistry analyses of Ab-1 or JSB indicated that tumor cells expressed MDR-1 protein in all three cell lines. Tc-99m MIBI is a good radiotracer for detecting drug resistance in lymphoma cell lines. (C) 2001 Elsevier Science Ireland Ltd. All rights reserved.
Relation:	SCANDINAVIAN JOURNAL OF RHEUMATOLOGY 30(4):225-228
Appears in Collections:	[China Medical University Hospital] Jurnal articles

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