Disabled-2 small interfering RNA modulates cellular adhesive function and MAPK activity during megakaryocytic differentiation of K562 cells

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題名:	Disabled-2 small interfering RNA modulates cellular adhesive function and MAPK activity during megakaryocytic differentiation of K562 cells
作者:	Tseng, CP;Huang, CL;Huang, CH;Cheng, JC;Stern, A;Tseng, CH;Chiu, DTY
貢獻者:	健康照護學院醫技系;Chang Gung Univ, Grad Inst Med Biotechnol, Tao Yuan 333, Taiwan;Natl Taiwan Univ, Dept Internal Med, Taipei 10764, Taiwan;NYU, Sch Med, Dept Pharmacol, New York, NY USA;China Med Coll, Dept Med Technol, Taichung, Taiwan;Chang Gung Univ, Grad Inst Basic Med Sci, Tao Yuan, Taiwan
日期:	2003
上傳時間:	2010-09-24 14:38:35 (UTC+8)
出版者:	ELSEVIER SCIENCE BV
摘要:	An efficient clean-up procedure coupled with a high performance liquid chromatography (HPLC) with on-line electrochemical (EC) reduction and fluorescence detection (FLD) was developed to quantify nitrated polycyclic aromatic hydrocarbons (NPAHs) in the airborne particulate. In this process, NPAHs were extracted ultrasonically followed by analysis by using a reversed phase column with an aqueous eluent containing 70% aqueous acetonitrile and sodium monocholoroacetate as a buffer solution. The extraction efficiencies were above 83% for 1-nitropyrene and 1,3-dinitropyrene (1,3-DNP) 1,6-DNP, and 1,8-DNP, and calibration graphs were linear with very good correlation coefficients (r > 0.999) and the detection limits were in the range of 1.0-2.2 pg for dinitropyrenes and nitropyrene. The proposed method provides a relatively simple and convenient procedure for determining the NPAHs samples in airborne particulate. (C) 2003 Elsevier Science B.V. All rights reserved.
關聯:	FEBS LETTERS 541(1月3日):21-27
顯示於類別:	[醫學檢驗生物技術學系暨碩士班 ] 期刊論文

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