Disabled-2 small interfering RNA modulates cellular adhesive function and MAPK activity during megakaryocytic differentiation of K562 cells

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Title:	Disabled-2 small interfering RNA modulates cellular adhesive function and MAPK activity during megakaryocytic differentiation of K562 cells
Authors:	Tseng, CP;Huang, CL;Huang, CH;Cheng, JC;Stern, A;Tseng, CH;Chiu, DTY
Contributors:	健康照護學院醫技系;Chang Gung Univ, Grad Inst Med Biotechnol, Tao Yuan 333, Taiwan;Natl Taiwan Univ, Dept Internal Med, Taipei 10764, Taiwan;NYU, Sch Med, Dept Pharmacol, New York, NY USA;China Med Coll, Dept Med Technol, Taichung, Taiwan;Chang Gung Univ, Grad Inst Basic Med Sci, Tao Yuan, Taiwan
Date:	2003
Issue Date:	2010-09-24 14:38:35 (UTC+8)
Publisher:	ELSEVIER SCIENCE BV
Abstract:	An efficient clean-up procedure coupled with a high performance liquid chromatography (HPLC) with on-line electrochemical (EC) reduction and fluorescence detection (FLD) was developed to quantify nitrated polycyclic aromatic hydrocarbons (NPAHs) in the airborne particulate. In this process, NPAHs were extracted ultrasonically followed by analysis by using a reversed phase column with an aqueous eluent containing 70% aqueous acetonitrile and sodium monocholoroacetate as a buffer solution. The extraction efficiencies were above 83% for 1-nitropyrene and 1,3-dinitropyrene (1,3-DNP) 1,6-DNP, and 1,8-DNP, and calibration graphs were linear with very good correlation coefficients (r > 0.999) and the detection limits were in the range of 1.0-2.2 pg for dinitropyrenes and nitropyrene. The proposed method provides a relatively simple and convenient procedure for determining the NPAHs samples in airborne particulate. (C) 2003 Elsevier Science B.V. All rights reserved.
Relation:	FEBS LETTERS 541(1月3日):21-27
Appears in Collections:	[Department of Medical Laboratory Science and Biotechnology ] Journal articles

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