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http://ir.cmu.edu.tw/ir/handle/310903500/29405
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| 題名: | Association between quantitative high-risk human papillomavirus DNA load and cervical intraepithelial neoplasm risk. |
| 作者: | Tsai, HT;Wu, CH;Lai, HL;Li, RN;Tung, YC;Chuang, HY;Wu, TN;Lin, LJ;Ho, CK;Liu, HW;Wu, MT |
| 貢獻者: | 附設醫院家醫科;Kaohsiung Med Univ, Grad Inst Basic Med, Dept Obstet & Gynaecol, Kaohsiung 807, Taiwan;Kaohsiung Med Univ, Grad Inst Basic Med, Dept Occupat & Environm Med, Kaohsiung 807, Taiwan;Kaohsiung Med Univ, Grad Inst Basic Med, Dept Family Med, Kaohsiung 807, Taiwan;Kaohsiung Municipal United Hosp, Tatung Hosp, Dept Nursing, Kaohsiung, Taiwan;I Shou Univ & Hosp, Dept Family Med, Kaohsiung, Taiwan;China Med Univ & Hosp, Dept Family Med, Taichung, Taiwan |
| 日期: | 2005 |
| 上傳時間: | 2010-09-24 14:33:57 (UTC+8) |
| 出版者: | AMER ASSOC CANCER RESEARCH |
| 摘要: | Previous studies have demonstrated that berberine decreases N-acetyltransferase (NAT) activity in human leukemia HL-60 cells, however, NAT gene expression has not been investigated. In this study, berberine was selected for testing the inhibition of N-acetylation of 2-aminofluorene (AF) and NAT gene expression in human HL-60 cells. The N-acetylation of AF was determined and quantitated by high performance liquid chromatography (HPLC). The data showed that a 24-hour berberine treatment decreased the amount of N-acetylation of AF in HL-60 cells. The NAT enzymes were stained and examined by Western blotting and flow cytometry. The results indicated that berberine decreased the levels of NAT protein in HL-60 cells. The expression of NAT gene (mRNAT NAT1) was determined by polymerase chain reaction (PCR), and it was found that berberine had inhibited the expression of mRNA NAT1 in human HL-60 cells. |
| 關聯: | CANCER EPIDEMIOLOGY BIOMARKERS & PREVENTION 14(11):2544-2549 |
| 顯示於類別: | [台中附設醫院] 期刊論文
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