Association between quantitative high-risk human papillomavirus DNA load and cervical intraepithelial neoplasm risk.

CMUR > China Medical University Hospital > Jurnal articles > Item 310903500/29405

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Title:	Association between quantitative high-risk human papillomavirus DNA load and cervical intraepithelial neoplasm risk.
Authors:	Tsai, HT;Wu, CH;Lai, HL;Li, RN;Tung, YC;Chuang, HY;Wu, TN;Lin, LJ;Ho, CK;Liu, HW;Wu, MT
Contributors:	附設醫院家醫科;Kaohsiung Med Univ, Grad Inst Basic Med, Dept Obstet & Gynaecol, Kaohsiung 807, Taiwan;Kaohsiung Med Univ, Grad Inst Basic Med, Dept Occupat & Environm Med, Kaohsiung 807, Taiwan;Kaohsiung Med Univ, Grad Inst Basic Med, Dept Family Med, Kaohsiung 807, Taiwan;Kaohsiung Municipal United Hosp, Tatung Hosp, Dept Nursing, Kaohsiung, Taiwan;I Shou Univ & Hosp, Dept Family Med, Kaohsiung, Taiwan;China Med Univ & Hosp, Dept Family Med, Taichung, Taiwan
Date:	2005
Issue Date:	2010-09-24 14:33:57 (UTC+8)
Publisher:	AMER ASSOC CANCER RESEARCH
Abstract:	Previous studies have demonstrated that berberine decreases N-acetyltransferase (NAT) activity in human leukemia HL-60 cells, however, NAT gene expression has not been investigated. In this study, berberine was selected for testing the inhibition of N-acetylation of 2-aminofluorene (AF) and NAT gene expression in human HL-60 cells. The N-acetylation of AF was determined and quantitated by high performance liquid chromatography (HPLC). The data showed that a 24-hour berberine treatment decreased the amount of N-acetylation of AF in HL-60 cells. The NAT enzymes were stained and examined by Western blotting and flow cytometry. The results indicated that berberine decreased the levels of NAT protein in HL-60 cells. The expression of NAT gene (mRNAT NAT1) was determined by polymerase chain reaction (PCR), and it was found that berberine had inhibited the expression of mRNA NAT1 in human HL-60 cells.
Relation:	CANCER EPIDEMIOLOGY BIOMARKERS & PREVENTION 14(11):2544-2549
Appears in Collections:	[China Medical University Hospital] Jurnal articles

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