Overall obesity and abdominal obesity and the risk of metabolic abnormalities

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Title:	Overall obesity and abdominal obesity and the risk of metabolic abnormalities
Authors:	Lai, SW;Ng, KC
Contributors:	附設醫院急診部;China Med Univ Hosp, Dept Emergency Med, Taichung 404, Taiwan;China Med Univ Hosp, Dept Family Med, Taichung, Taiwan
Date:	2004
Issue Date:	2010-09-24 14:33:11 (UTC+8)
Publisher:	ROYAL ACADEMY MEDICINE
Abstract:	The phage-displayed combinatorial peptide library is a revolutionary method for discovering epitopes, in particular conformational epitopes. In this study, we characterized a Japanese encephalitis virus (JEV) conformational epitope by biopanning of phage-displayed random peptide libraries with a JEV envelope (E) protein-specific monoclonal antibody (mAb) 2H2. Eleven identified phage clones with high affinity to mAb 2H2 were identified using direct and inhibitory binding ELISA. Sequence alignment, structure modeling and mutational analysis revealed that the identified mimotopes for mAb 2H2 possess a conserved motif X-1(D/E)(Y/T/S)X-2, fitting into a region at the domain III lateral surface of the E protein. The results of our study could provide useful information on the development of effective mimotope-based vaccines and diagnostic kits for the JEV infection. Copyright (C) 2004 S. Karger AG, Basel.
Relation:	IRISH JOURNAL OF MEDICAL SCIENCE 173(4):193-196
Appears in Collections:	[China Medical University Hospital] Jurnal articles

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