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請使用永久網址來引用或連結此文件:
http://ir.cmu.edu.tw/ir/handle/310903500/28762
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| 題名: | Relationship between antibacterial activity of chitosan and surface characteristics of cell wall |
| 作者: | Chung, YC;Su, YP;Chen, CC;Jia, G;Wang, HI;Wu, JCG;Lin, JG |
| 貢獻者: | 中醫學院中醫所;China Inst Technol, Dept Ind Engn & Management, Taipei 245, Taiwan;Natl Taiwan Normal Univ, Dept Chem, Taipei 116, Taiwan;Natl Taichung Nursing Coll, Dept Gen Studies, Taichung 403, Taiwan;Peking Univ, Sch Publ Hlth, Dept Occupat & Environm Hlth Sci, Beijing 100083, Peoples R China;Fooyin Univ, Basic Med Sci Educ Ctr, Kaohsiung 831, Taiwan;China Med Univ, Inst Chinese Med Sci, Taichung 404, Taiwan |
| 日期: | 2004 |
| 上傳時間: | 2010-09-24 13:45:27 (UTC+8) |
| 出版者: | ACTA PHARMACOLOGICA SINICA |
| 摘要: | In this study, we have evaluated the chemopreventive role of aloe-emodin in human promyelocytic leukemia HL-60 cells in vitro by studying the regulation of proliferation, cell cycle and apoptosis. Aloe-emodin inhibited cell proliferation and induced G2/M arrest and apoptosis in HL-60 cells. Investigation of the levels of cyclins B1, E and A by immunoblot analysis showed that cyclin E level was unaffected, whereas cyclin B I and A levels increased with aloe-emodin in HL-60 cells. Investigation of the levels of cyclin-dependent kinases, CdkI and 2, showed increased levels of Cdk1 but the levels of Cdk2 were not effected with aloe-emodin in HL-60 cells. The levels of p27 were increased after HL-60 cells were cotreated with various concentrations of aloe-emodin. The increase of the levels of p27 may be the major factor for aloe-emodin to cause G2/M arrest in these examined cells. Flow cytometric assays and DNA fragmentation gel electrophoresis also confirmed aloe-emodin induced apoptosis in HL-60 cells. The levels of caspase-3 were increased after HL-60 cells were cotreated with 10 M aloe-emodin for 12, 24, 48, and 72 hours. Taken together, aloe-emodin therefore appears to exert its anticarcinogenesis properties by inhibiting proliferation and inducing cell cycle arrest and apoptosis underwent activation of caspase-3 in human leukemia HL-60 cells. (C) 2004 Elsevier Ltd. All rights reserved. |
| 關聯: | ACTA PHARMACOLOGICA SINICA 25(7):932-936 |
| 顯示於類別: | [中國醫學研究所] 期刊論文
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