Relationship between antibacterial activity of chitosan and surface characteristics of cell wall

中國醫藥大學機構典藏 China Medical University Repository, Taiwan > 中醫學院 > 中國醫學研究所 > 期刊論文 > Item 310903500/28762

jsp.display-item.identifier=請使用永久網址來引用或連結此文件: http://ir.cmu.edu.tw/ir/handle/310903500/28762

题名:	Relationship between antibacterial activity of chitosan and surface characteristics of cell wall
作者:	Chung, YC;Su, YP;Chen, CC;Jia, G;Wang, HI;Wu, JCG;Lin, JG
贡献者:	中醫學院中醫所;China Inst Technol, Dept Ind Engn & Management, Taipei 245, Taiwan;Natl Taiwan Normal Univ, Dept Chem, Taipei 116, Taiwan;Natl Taichung Nursing Coll, Dept Gen Studies, Taichung 403, Taiwan;Peking Univ, Sch Publ Hlth, Dept Occupat & Environm Hlth Sci, Beijing 100083, Peoples R China;Fooyin Univ, Basic Med Sci Educ Ctr, Kaohsiung 831, Taiwan;China Med Univ, Inst Chinese Med Sci, Taichung 404, Taiwan
日期:	2004
上传时间:	2010-09-24 13:45:27 (UTC+8)
出版者:	ACTA PHARMACOLOGICA SINICA
摘要:	In this study, we have evaluated the chemopreventive role of aloe-emodin in human promyelocytic leukemia HL-60 cells in vitro by studying the regulation of proliferation, cell cycle and apoptosis. Aloe-emodin inhibited cell proliferation and induced G2/M arrest and apoptosis in HL-60 cells. Investigation of the levels of cyclins B1, E and A by immunoblot analysis showed that cyclin E level was unaffected, whereas cyclin B I and A levels increased with aloe-emodin in HL-60 cells. Investigation of the levels of cyclin-dependent kinases, CdkI and 2, showed increased levels of Cdk1 but the levels of Cdk2 were not effected with aloe-emodin in HL-60 cells. The levels of p27 were increased after HL-60 cells were cotreated with various concentrations of aloe-emodin. The increase of the levels of p27 may be the major factor for aloe-emodin to cause G2/M arrest in these examined cells. Flow cytometric assays and DNA fragmentation gel electrophoresis also confirmed aloe-emodin induced apoptosis in HL-60 cells. The levels of caspase-3 were increased after HL-60 cells were cotreated with 10 M aloe-emodin for 12, 24, 48, and 72 hours. Taken together, aloe-emodin therefore appears to exert its anticarcinogenesis properties by inhibiting proliferation and inducing cell cycle arrest and apoptosis underwent activation of caspase-3 in human leukemia HL-60 cells. (C) 2004 Elsevier Ltd. All rights reserved.
關聯:	ACTA PHARMACOLOGICA SINICA 25(7):932-936
显示于类别:	[中國醫學研究所] 期刊論文

文件中的档案:

没有与此文件相关的档案.

在CMUR中所有的数据项都受到原著作权保护.

TAIR相关文章

数据加载中.....