The effect of fasting on the pulse spectrum

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题名:	The effect of fasting on the pulse spectrum
作者:	Su, YC;Huang, KF;Chang, YH;Li, TC;Huang, WS;Lin, JG
贡献者:	中醫學院中醫所;China Med Coll, Inst Chinese Med Sci, Basic Chinese Med Lab, Taichung, Taiwan
日期:	2000
上传时间:	2010-09-24 13:44:12 (UTC+8)
出版者:	INST ADVANCED RESEARCH ASIAN SCIENCE & MEDICINE, INC
摘要:	A recombinant plasmid, which contains the Pseudomonas aeruginosa exotoxin A (PE) gene with a C-terminal deletion, was inserted into expression vector pSecTag Xpress. The expression of this bacterial exotoxin in an animal cell was first demonstrated in 3T3 cell by transient transfection and western blot assay. Recombinant plasmid DNA was then injected intramuscularly to BALB/c mice, anti-PE specific antibodies were found in all animals vaccinated with plasmid containing the PE gene and with 'detoxicated' recombinant PE protein. Mice vaccinated with DNA were protected from the intoxication of lethal dosage of P. aeruginosa exotoxin A. Our results indicated that mice vaccinated with DNA encoding the PE gene could express PE protein in vivo, induced specific immune response, and provided sufficient protective immunity that safeguarded mice from the injection of lethal dosage of PE toxin. (C) 2000 Elsevier Science Ltd. All rights reserved.
關聯:	AMERICAN JOURNAL OF CHINESE MEDICINE 28(3月4日):409-417
显示于类别:	[中國醫學研究所] 期刊論文

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