The effect of fasting on the pulse spectrum

CMUR > College of Chinese Medicine > Graduate Institute of Chinese Medical Science > Journal articles > Item 310903500/28727

Please use this identifier to cite or link to this item: http://ir.cmu.edu.tw/ir/handle/310903500/28727

Title:	The effect of fasting on the pulse spectrum
Authors:	Su, YC;Huang, KF;Chang, YH;Li, TC;Huang, WS;Lin, JG
Contributors:	中醫學院中醫所;China Med Coll, Inst Chinese Med Sci, Basic Chinese Med Lab, Taichung, Taiwan
Date:	2000
Issue Date:	2010-09-24 13:44:12 (UTC+8)
Publisher:	INST ADVANCED RESEARCH ASIAN SCIENCE & MEDICINE, INC
Abstract:	A recombinant plasmid, which contains the Pseudomonas aeruginosa exotoxin A (PE) gene with a C-terminal deletion, was inserted into expression vector pSecTag Xpress. The expression of this bacterial exotoxin in an animal cell was first demonstrated in 3T3 cell by transient transfection and western blot assay. Recombinant plasmid DNA was then injected intramuscularly to BALB/c mice, anti-PE specific antibodies were found in all animals vaccinated with plasmid containing the PE gene and with 'detoxicated' recombinant PE protein. Mice vaccinated with DNA were protected from the intoxication of lethal dosage of P. aeruginosa exotoxin A. Our results indicated that mice vaccinated with DNA encoding the PE gene could express PE protein in vivo, induced specific immune response, and provided sufficient protective immunity that safeguarded mice from the injection of lethal dosage of PE toxin. (C) 2000 Elsevier Science Ltd. All rights reserved.
Relation:	AMERICAN JOURNAL OF CHINESE MEDICINE 28(3月4日):409-417
Appears in Collections:	[Graduate Institute of Chinese Medical Science] Journal articles

Files in This Item:

There are no files associated with this item.