This research project attempts to create Streptomycin derivative by glutaldehde method and then conjugate this derivative to a protein for immunization of animal to produce antibody against streptomycin.The conjugation of streptomycin derivative to enzyme, HRP, will also be carried out for the titration of antibody and establishment of ELISA.Erythromycin will be converted to derivative by oxime method by utilizing two keto groups in the erythromycin.The derivative will then be conjugated to protein for immunization and to enzyme, HRP, for titration of antibody and formulation of ELISA kits.The validation of ELISA for both streptomycin and erythromycin will be carried according to our past experience in establishing standard curve, sensitivity determination, cross-reactivity test, recovery rate and utilizing HPLC and/or capillary electrophoresis to verify and validate the ELISA.
