Long-chained nonylphenol ethoxylates (NPEOs) are widely used in nonion surfactants such as detergents.When long-chained NPEOs are discharged, they become gradually short-chained ones due to biodegradation. The final product is nonylphenol (NP), which has been proved as one of environmental hormones. People make many NPEOs household cleaners whose sewage is inadequately treated, so NP is detected in rivers. It has been threatening the environment and human seriously. NP, whose precursors are NPEOs, is usually manufactured for industrial use such as sticky stuff, resin and plastic. Under anaerobic condition, NPEOs will transform NP, which is a kind of persistent. By food chain, NP causes reproductive damage. People come into contact with NPEOs goods easily in daily life. Therefore Environmental Protection Administration (EPA) has revised detergent environmental labeling criteria, which is lower than 0.1%. In the other word, NP management is the main point for environmental protection in the future. Nowadays, improved NP hydrolysis in urine is hydrochloric acid method. We will reform this method and apply it to sludge preparation. NPEOs can convert NP by acid. After that, NP will be analyzed for qualitative and quantity with HPLC/ fluorescence detector. For this study, hydrochloric acid preparation will be to assess NP/NPEOs ratio in wastewater treatment plant (WWTP). "
