Analysis of flavonoids in Vernonia paltula by high-performance liquid chromatography

中國醫藥大學機構典藏 China Medical University Repository, Taiwan > 藥學院 > 中國藥學研究所(已停用) > 期刊論文 > Item 310903500/30744

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题名:	Analysis of flavonoids in Vernonia paltula by high-performance liquid chromatography
作者:	Ku, YR;Chen, CY;Ho, LK;Lini, JH;Chang, YS
贡献者:	藥學院中藥所;Natl Labs Foods & Drugs, Dept Hlth, Execut Yuan, Taipei 115, Taiwan;China Med Coll, Inst Chinese Pharmaceut Sci, Taichung 404, Taiwan;Natl Yang Ming Univ, Dept Pharmacol, Taipei 112, Taiwan;Natl Yang Ming Univ, Inst Pharmacol, Taipei 112, Taiwan
日期:	2002
上传时间:	2010-09-24 15:01:00 (UTC+8)
出版者:	NATL LABORATORIES FOODS DRUGS
摘要:	Hesperidin and hesperetin are flavanones in many Citrus fruits with antiulcer, antioxidation and anticancer activities. Hesperetin is the aglycone and a metabolite of hesperidin. HPLC analysis was developed for the determination of hesperetin and its conjugate metabolites in serum and urine prior to and after hydrolysis following oral administrations of hesperetin, hesperidin, or decoction of Pericarpium Citri Reticulatae (Chenpi). The hydrolysis was conducted away from light by using glucuronidase/sulfatase in the presence of ascorbic acid at 37degreesC. The HPLC methods employed a LiChrospher 100 RP-18e column, using acetonitrile/1% acetic acid (36/65) and methanol/acetonitrile/1% acetic acid (17/26/57) as mobile phases for serum and for urine, respectively. For internal standard, 5,7-dimethoxycoumarin was used. The present methods are rapid, sensitive, reproducibility, precise, and accurate with good recoveries. They are applicable for the pharmacokinetic studies of hesperidin and hesperetin as well as Citrus foods or herbs containing them.
關聯:	JOURNAL OF FOOD AND DRUG ANALYSIS 10(3):139-142
显示于类别:	[中國藥學研究所(已停用)] 期刊論文

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