Analysis of flavonoids in Vernonia paltula by high-performance liquid chromatography

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Title:	Analysis of flavonoids in Vernonia paltula by high-performance liquid chromatography
Authors:	Ku, YR;Chen, CY;Ho, LK;Lini, JH;Chang, YS
Contributors:	藥學院中藥所;Natl Labs Foods & Drugs, Dept Hlth, Execut Yuan, Taipei 115, Taiwan;China Med Coll, Inst Chinese Pharmaceut Sci, Taichung 404, Taiwan;Natl Yang Ming Univ, Dept Pharmacol, Taipei 112, Taiwan;Natl Yang Ming Univ, Inst Pharmacol, Taipei 112, Taiwan
Date:	2002
Issue Date:	2010-09-24 15:01:00 (UTC+8)
Publisher:	NATL LABORATORIES FOODS DRUGS
Abstract:	Hesperidin and hesperetin are flavanones in many Citrus fruits with antiulcer, antioxidation and anticancer activities. Hesperetin is the aglycone and a metabolite of hesperidin. HPLC analysis was developed for the determination of hesperetin and its conjugate metabolites in serum and urine prior to and after hydrolysis following oral administrations of hesperetin, hesperidin, or decoction of Pericarpium Citri Reticulatae (Chenpi). The hydrolysis was conducted away from light by using glucuronidase/sulfatase in the presence of ascorbic acid at 37degreesC. The HPLC methods employed a LiChrospher 100 RP-18e column, using acetonitrile/1% acetic acid (36/65) and methanol/acetonitrile/1% acetic acid (17/26/57) as mobile phases for serum and for urine, respectively. For internal standard, 5,7-dimethoxycoumarin was used. The present methods are rapid, sensitive, reproducibility, precise, and accurate with good recoveries. They are applicable for the pharmacokinetic studies of hesperidin and hesperetin as well as Citrus foods or herbs containing them.
Relation:	JOURNAL OF FOOD AND DRUG ANALYSIS 10(3):139-142
Appears in Collections:	[Graduate Institute of Chinese Pharmaceutical Science] Journal articles

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