Effects of platelet activating factor, butyrate and interleukin-6 on cyclooxygenase-2 expression in human esophageal cancer cells

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Title:	Effects of platelet activating factor, butyrate and interleukin-6 on cyclooxygenase-2 expression in human esophageal cancer cells
Authors:	Wang, LS;Chow, KC;Wu, YC
Contributors:	附設醫院醫研部;Taipei Vet Gen Hosp, Dept Surg, Div Thorac Surg, Taipei 112, Taiwan;China Med Coll Hosp, Dept Med Res, Taichung, Taiwan;Natl Yang Ming Univ, Taipei 112, Taiwan
Date:	2002
Issue Date:	2010-09-24 14:57:07 (UTC+8)
Publisher:	TAYLOR & FRANCIS AS
Abstract:	Glycyrrhizin, a major component of Glycyrrhiza uralensis (licorice) root, is a saponin and exhibits a number of pharmacological effects, including anti-inflammation, anti-ulcer, anti-allergy, and anti-carcinogenesis. Activator protein 1 (AP-1), a nuclear transcription factor, consists of Jun/Fos heterodimers or Jun/Jun homodimers, and blocking of tumor promoter-induced AP-1 activity could inhibit induced cellular transformation. In order to elucidate the molecular mechanism of glycyrrhizin-induced antic arcinogenesis, effect of glycyrrhizin on the AP-1 activity in untreated and tumor promoter-12-O-tetradecanoylphorbol-13-acetate (TPA)-treated conditions was analyzed in this study. Glycyrrhizin induced the AP-1/TATA reporter activity in a dose-dependent fashion, which was judged by chloramphenicol acetyltransferase assay and electrophoretic mobility-shift assay. Similar results were observed in HepG2 and Vero cells, suggested that glycyrrhizin effect was cell type-independent. In addition, the cis element responsible for glycyrrhizin activity was AP-1 responsive element. Further analysis indicated that glycyrrhizin exhibited a different regulation on the AP-1 activity in untreated and TPA-treated cells. Glycyrrhizin induced the AP-1 activity in untreated cells, while it inhibited the TPA-induced AP-1 activation in TPA-treated cells. These results provide insight into the biological actions of glycyrrhizin and the molecular basis for the development of new chemoprotective agents for cancer. (C) 2002 Elsevier Science Inc. All rights reserved.
Relation:	SCANDINAVIAN JOURNAL OF GASTROENTEROLOGY 37(4):467-475
Appears in Collections:	[China Medical University Hospital] Jurnal articles

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