Accuracy of three diagnostic tests used alone and in combination for detecting Helicobacter pylori infection in patients with bleeding gastric ulcers

中國醫藥大學機構典藏 China Medical University Repository, Taiwan > 醫學院 > 醫學系 > 期刊論文 > Item 310903500/30166

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题名:	Accuracy of three diagnostic tests used alone and in combination for detecting Helicobacter pylori infection in patients with bleeding gastric ulcers
作者:	Liao, CC;Lee, CL;Lai, YC;Huang, SH;Lee, SC;Wu, CH;Tu, TC;Chen, TK;Bai, CH
贡献者:	醫學院醫學系;Cathay Gen Hosp, Div Gastroenterol, Dept Internal Med, Taipei 106, Taiwan;Cathay Gen Hosp, Div Pathol, Taipei 106, Taiwan;China Med Univ, Sch Med, Taichung 400, Taiwan;Taipei Med Univ, Sch Med, Taipei 222, Taiwan;Inst Nucl Energy Res, Taoyuan 300, Taiwan;Acad Sinica, Dept Epidemiol, Inst Biomed Sci, Taipei 106, Taiwan
日期:	2003
上传时间:	2010-09-24 14:50:26 (UTC+8)
出版者:	CHINESE MEDICAL ASSOCIATION
摘要:	The aim of this study was to investigate the effect of electrical stimulation on activator protein-1 (AP-1) in recombinant liver cells. In order to elucidate the molecular effects of electrical stimulation on cells, AP-1 expression was detected by a luciferase assay. The parameters used were taken from clinical electroacupuncture (EA) therapy as follows: biphasic rectangular symmetrical pulses (frequency: 2, 10 and 100 Hz; pulse width: 50, 80, 130 and 250 musec; intensity: 1, 2, 5 and 10 mA; time: 5, 10, 20 and 30 minutes). S (10% fetal bovine serum in medium), SF(serum free medium) and 12-O-tetradecanoylphorbol-13-acetate (TPA) treatment represented the experimental, negative and positive groups, respectively. We found that electrical stimulation with 10 Hz, a pulse width of 130 musec, and a duration of 30 minutes gave a significant increase in AP-1 activity. In contrast, the intensity of the stimulation had no significant effect on AP-1 activity. In conclusion, our data suggest that electrical stimulation with an appropriate frequency, duration and pulse width could cause an increase in AP-1 activity in cells.
關聯:	CHINESE MEDICAL JOURNAL 116(12):1821-1826
显示于类别:	[醫學系] 期刊論文

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