Parkinson’s disease is a progressive neurodegenerative disorder. Many researches reveal the overproduction of free radical caused by oxidative stress plays an important role in this disease. In this study, the antioxidant components, antioxidative activity of five species of Davalliaceae plants and two polypodiaceae plants were investigated first by microtiter spectrophotometric and fluorescent methods. Then the protective effects of these plants on 6-OHDA-induced neuronal damage in B35 cells were investigated.
The experimental results suggested that Davallia mariesii and Araiostegia parvipinnata have enriched antioxidant components and better antioxidative activity. However, Davallia divaricata and Davallia mariesii had better hydroxyl radical scavenging activity than the others. Furthermore, Davallia mariesii, Drynaria fortunei and Araiostegia parvipinnata had better inhibitive capability in the production of p-quinone from 6-OHDA.
Davallia mariesii has the hightest protective effects among the aqueous extracts of these three plants on the neuronal oxidative damage induced by 6-OHDA in B35 neuroblastoma cell. The neuroprotective mechanism of Davallia mariesii might be due to its phenolic antioxidative activity, and efficiently decreased intracellular oxidative stress and damage induced by 6-OHDA.
