中國醫藥大學機構典藏 China Medical University Repository, Taiwan:Item 310903500/880
English  |  正體中文  |  简体中文  |  全文笔数/总笔数 : 29490/55136 (53%)
造访人次 : 1524188      在线人数 : 350
RC Version 7.0 © Powered By DSPACE, MIT. Enhanced by NTU Library IR team.
搜寻范围 查询小技巧：
  • 您可在西文检索词汇前后加上"双引号"，以获取较精准的检索结果
  • 若欲以作者姓名搜寻，建议至进阶搜寻限定作者字段，可获得较完整数据
    •  进阶搜寻
    主页 ‧ 登入 ‧ 上传 ‧ 说明 ‧ 关于CMUR ‧ 管理 到手机版


    jsp.display-item.identifier=請使用永久網址來引用或連結此文件: http://ir.cmu.edu.tw/ir/handle/310903500/880


    题名: 苯甲基異硫氰酸酯和苯乙基異硫氰酸酯會誘導人類前列腺癌細胞株(DU-145)細胞週期停滯和造成細胞凋亡;Benzyl Isothiocyanate and Phenethyl Isothiocyanate Induce Cell Cycle Arrest and Apoptosis in Human Prostate Cancer DU-145 Cells
    作者: 黃雅婷;Ya-Ting Huang
    贡献者: 中國醫藥大學：醫學檢驗生物技術學系碩士班
    关键词: 苯甲基異硫氰酸酯;苯乙基異硫氰酸酯;細胞凋亡;細胞週期停滯;BITC;PEITC;Apoptosis
    日期: 2009-05-26
    上传时间: 2009-08-12 17:09:57 (UTC+8)
    摘要: 苯甲基異硫氰酸酯和苯乙基異硫氰酸酯是來自於十字花科蔬菜的衍生物，常見的十字花科蔬菜包含了花椰菜、甘藍菜、芥菜…等。已有文獻指出苯甲基異硫氰酸酯會造成多種人類癌細胞株的凋亡，例如：卵巢癌細胞、乳癌細胞和胰臟癌細胞。另外，也有文獻指出苯乙基異硫氰酸酯會造成人類非小細胞肺癌和前列腺癌細胞(PC-3)的凋亡。但是苯甲基異硫氰酸酯和苯乙基異硫氰酸酯造成人類前列腺癌細胞株DU-145的凋亡機轉則尚未有較詳細的報導，所以本研究即是探討苯甲基異硫氰酸酯和苯乙基異硫氰酸酯造成人類前列腺癌細胞株DU-145的相關凋亡機轉。由結果顯示，苯甲基異硫氰酸酯和苯乙基異硫氰酸酯確實會造成人類前列腺癌細胞株DU-145細胞週期停滯以及誘導細胞凋亡的產生。除此之外，苯甲基異硫氰酸酯和苯乙基異硫氰酸酯也會造成人類前列腺癌細胞株DU-145粒線體膜電位的改變，活性氧化物質和鈣離子的釋放以及造成DNA的損傷。接著，由西方點墨法的結果發現苯甲基異硫氰酸酯和苯乙基異硫氰酸酯會造成人類前列腺癌細胞株DU-145凋亡蛋白的增加和Caspase活性的上升。由此可知，苯甲基異硫氰酸酯和苯乙基異硫氰酸酯會造成人類前列腺癌細胞株DU-145走向凋亡的命運，值得更進一步研究發展為抗癌藥物。

    Benzyl isothiocyanate and phenethyl isothiocyanate, are derived isothiocyanates from cruciferous vegetable, including watercress, broccoli and cabbage. Benzyl isothiocyanate has been shown that to induce cell cycle arrest and apoptosis in many human cancer cell lines, such as ovarian cancer cells, breast cancer MDA-MB-231 and MCF-7 cells and pancreatic cancer Capan-2 cells. Phenethyl isothiocyanate also has been shown that to induce cell cycle arrest and apoptosis in many human cancer cell lines, such as human non-small lung cancer cells, prostate cancer PC-3 cells (Prostate cancer metastasized to bone). In this study, we investigate the effect of benzyl isothiocyanate and phenethyl isothiocyanate on human prostate cancer DU-145 cells, the results demonstrate that benzyl isothiocyanate and phenethyl isothiocyanate induced cause G2 / M phase arrest and apoptosis. These effects are in a dose- and time-dependent manners. Besides, benzyl isothiocyanate and phenethyl isothiocyanate also decreased the levels of mitochondria membrane potential, induced reactive oxygen species and calcium ion production in examined DU-145 cells. Benzyl isothiocyanate and phenethyl isothiocyanate induced apoptosis of DU-145 cells which also were confirmed by DAPI staining and DNA gel electrophoresis. Western blotting show that benzyl isothiocyanate and phenethyl isothiocyanate treatment gradually decreased the level of anti-apoptotic proteins, but increased the level of pro-apoptotic proteins. Besides, benzyl isothiocyanate and phenethyl isothiocyanate increased caspase-3 activity. Therefore, benzyl isothiocyanate and phenethyl isothiocyanate induce apoptosis in human prostate cancer DU-145 cells.
    显示于类别:[醫學檢驗生物技術學系暨碩士班 ] 博碩士論文

    文件中的档案:

    档案 描述 大小格式浏览次数
    cmu-98-9673856-1.pdf2843KbAdobe PDF405检视/开启
    index.html0KbHTML157检视/开启


    在CMUR中所有的数据项都受到原著作权保护.

    TAIR相关文章

     

    DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library IR team Copyright ©   - 回馈