Analysis of Bioactive Triterpenes in Eriobotrya japonica LINDL. by High-Performance Liquid Chromatography

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Title:	Analysis of Bioactive Triterpenes in Eriobotrya japonica LINDL. by High-Performance Liquid Chromatography
Authors:	何惠雅(HUI-YA HO);林文川(Wen-Chuan Lin);北中進(SUSUMU KITANAKA);張淳堆(Chwen-Tzuei Chang);吳金濱(Jin Bin Wu)*
Contributors:	藥學院藥物化學研究所
Keywords:	Eriobotrya japonica;HPLC;tormentic acid;maslinic acid;corosolic acid;oleanolic acid;ursolic acid
Date:	2008-12
Issue Date:	2009-08-26 16:28:31 (UTC+8)
Abstract:	Eriobotrya japonica (Rosaceae) has been used for clearing away heat in the lung to stop cough and lowering the adverse flow of gi to stop vomiting. High-performance liquid chromatographic method was applied to assay the five triterpenes contents in E. japonica from various locations. Samples were analyzed on a HyPURITY C-18 column (5 μm, 4.6 × 250 mm) eluted at a rate of 0.5 mL/min with a solvent of A - B (A, Methanol; B, 0.15% acetic acid aqueous; A: B = 85: 15, v/v). Triterpenes were detected by refractive index detection (RID). Regression equations of the calibration curves revealed good linear relationships (correlation coefficients: 0.9998-1.0000). The recoveries ranged between 98.39% - 102.41% and indicated good accuracy.
Relation:	JOURNAL OF FOOD AND DRUG ANALYSIS 16(6)41 ~45
Appears in Collections:	[Graduate Institute of Pharmaceutical Chemistry] Journal articles

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