Crude Extracts of Solanum lyratum Induced Cytotoxicity and Apoptosis in a Human Colon Adenocarcinoma Cell Line (Colo 205)

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Title:	Crude Extracts of Solanum lyratum Induced Cytotoxicity and Apoptosis in a Human Colon Adenocarcinoma Cell Line (Colo 205)
Authors:	徐素琴(Shu-Chun Hsu);JIH-HUNG LU;郭昭麟(Kuo Chao-Lin)*;楊家欣(Yang Jai-Sing);(Lin MW);陳光偉(Chen GW);蘇進誠(Su CC);呂旭峰(Lu HF);鍾景光(Jing-Gung Chung)
Contributors:	藥學院中藥資源學系
Keywords:	Solanum lyratum extracts;cytotoxicity;apoptosis;colon adenocarcinoma cell lines
Date:	2008-05
Issue Date:	2009-08-26 16:23:22 (UTC+8)
Abstract:	The effects of the crude extract of Solanum lyratum (SLE) on human colon cancer colo 205 cells were investigated. The cell viability, morphological changes of the cells, cell cycle arrest, apoptosis, reactive oxygen species (ROS), mitochondrial membrane potential (Δæm) and cell cycle- and apoptosis-associated protein levels and gene expressions were examined in colo 205 cells after exposure to various concentrations of SLE for different time periods. The results indicated that SLE decreased the percentage of viable colo 205 cells accompanied by morphological changes. The most effective concentration of SLE was 300 μg/ml (SLE 300) and this concentration was used for further investigations. SLE induced S-phase arrest and apoptosis (sub-G1) in the colo 205 cells and those effects were dose- and time-dependent. DAPI staining and DNA gel electrophoresis confirmed that SLE induced apoptosis in colo 205 cells. Flow cytometric analysis also showed that SLE 300 promoted ROS production and decreased the Δæm. Western blotting analysis indicated that SLE 300 increased Bax levels and decreased Bcl-2 levels, which caused the loss of Δæm followed by cytochrome c release and caspase-9 and -3 activation, finally leading to apoptosis. SLE 300 also promoted p53 and p27, but decreased the levels of cyclin B1 thus causing S-phase arrest. The gene expression associated with those proteins was also confirmed by PCR methods. The findings show that SLE might be used as a colon cancer therapeutic agent in the future.
Relation:	ANTICANCER RESEARCH 28(2A )1045 ~1054
Appears in Collections:	[Department of Chinese Medicine Resources] Journal articles

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