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| 題名: | Antioxidant and Free Radical Scavenging Activities of Phellinus merrillii Extracts |
| 作者: | (HY Chang);(YL Ho);(MJ Sheu);(YH Lin);(MC Tseng);(SH Wu);黃冠中(Huang Guan-jhong);張永勳(Yuan Shiun Chang)* |
| 貢獻者: | 藥學院中國藥學研究所;中國附醫藥劑部中藥局 |
| 關鍵詞: | 1, 1二苯基2 picrylhydrazyl DPPH,三價鐵還原性抗氧化力測試,自由基,谷胱甘肽,高效液相層析串聯式質譜分析法,梅里爾針層孔菌,清除活性,總抗氧化力,總酚類含量,Trolox 相等性的抗氧化力;1,1-dipheny1-2-picrylhydrazyl DPPH,Ferric reducing antioxidant power assay FRAP,Free radicals,Glutathione reduced form GSH,High-performance liquid chromatography tandem mass LC/MS/MS,Phellinus merrillii PM,Scavenging effect,Total antioxidant capacity,Total phenolics contents TPC,Trolox equivalent antioxidant capacity TEAC |
| 日期: | 2007-12 |
| 上傳時間: | 2009-08-26 16:21:40 (UTC+8) |
| 摘要: | 本研究主要的目的是研究梅里爾針層孔菌不同溶劑分層的萃取物之抗氧化活性。探討的方法如下：ABTS自由基清除測定、測定總酚類含量、FRAP測試、快速DPPH（1, 1二苯基 2 picrylhydrazyl）呈色反應、DPPH清除自由基活性和還原力等幾項。ABTS自由基清除測定中，在n-BuOH分層中，表現出最高的總抗氧化活性(17.13±0.04 mM)。測定總酚類含量和FRAP分析中，在EtOAc分層中，有最高的酚類含量(46.21±0.02 mM)和還原性抗氧化力(19.09±0.03mM)。在快速DPPH呈色反應中，在n-BuOH分層中，表現出最強的呈色反應。在DPPH清除自由基活性中，EtOAc分層中，有最強的抗氧化活性，其IC50為(0.66±0.01 mg/ml)。在還原力的測試中，粗抽出物中，在 2 mg/ml 濃度中有最高的還原力。在ABTS測試、FRAP測試和總酚類含量相互之間的比較時其相關係數是有意義的。在所有不同溶劑分層之萃取物之中，在EtOAc 有最高的總酚類含量。由結果可顯示梅里爾針層孔菌在EtOAc、n-BuOH分層中具有較高的抗氧化活性。在高效液相層析質譜儀分析法中，梅里爾針層孔菌的抽出物的指紋圖譜被建立，hispolon與梅里爾針層孔菌的抽出物顯示出有相似的波峰與相同的4.7分鐘的滯留時間，均獲得m/z 219與m/z 135較大的裂片，這表示梅里爾針層孔菌的乙醇抽出物含有活性成份hispolon。而hispolon和BHT的DPPH自由基清除之IC50為 42.4±2.9 和 81.2±3.2 μM，由此發現hispolon對於抗氧化活性是非常重要的，因此表示hispolon在梅里爾針層孔菌中有助於抗氧化活性。
This study aimed to investigate possible antioxidant activity of various extracts of Phellinus merrillii (PM). The explored items include: ABTS free radical scavenging assay, determination of total phenolics contents (TPC), ferric reducing antioxidant power assay (FRAP), rapid screening of antioxidant by dot-blot DPPH (1, 1-diphenyl-2-picrylhydrazyl) staining, DPPH radical-scavenging activities and reducing power measurement. In the ABTS free radical scavenging assay, the n-BuOH fraction displayed the highest total antioxidant activity (17.13 ± 0.04 mM). In the determination of total phenolics contents (TPC) and ferric reducing antioxidant power assay (FRAP), the EtOAc fraction had the highest phenolics contents (46.21±0.02 mM) and reducing antioxidant power (19.09±0.03 mM). In the rapid screening of antioxidant by dotblot DPPH staining, the n-BuOH fraction showed the highest strong dot-blot staining. In the reducing power measurement, the crude extract had the highest reducing power at 2 mg/ml concentration. In the DPPH radical-scavenging activities, the EtOAc fraction had the highest antioxidant activity (IC50=0.66±0.01 mg/ml). As regard the correlation coefficients among ABTS assay, FRAP assay, and total phenolics contents, it can be seen that correlation coefficients in each case were significant. Among all extracts, the highest amountof total phenolics contents were found in the EtOAc fractiont. It is suggested that the PM might ontribute its antioxidant activities on EtOAc and n-BuOH fraction. In high-performance liquid chromatography tandem mass (LC/MS/MS) analysis for hispolon, the daughter ion scanned chromatograms of PM was established. Both hispolon and PM showed similar daughter ion spectrum at the retention time of 4.7 min and had more lobes in m/z 219 and m/z 135. This indicated that PM did contain the active ingredient hispolon. Both the IC50 of DPPH radical scavenging activity for hispolon and BHT were 42.4±2.9 and 81.2 ± 3.2 μM, respectively. These findings mean that hispolon was most important in antiradical activities. It was suggested that hispolon might contribute to its antioxidant activities in PM. |
| 關聯: | Botanical Studies 48(4)407~417 |
| 顯示於類別: | [中國藥學研究所(已停用)] 期刊論文
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