Huai-niu-xi is the dried root of Achyranthes bidentata (Amaranthaceae) and a commonly used herbal medicine. It possesses analgesic, anti-inflammatory, diuretic, anti-bacterial, and anti-convulsive activity. In clinical use, it was used to strengthen the sinew and bones, supplement the liver and kidney, and cure knee pain. A simple, rapid, and accurate high-performance liquid chromatographic (HPLC) method was developed for the assay of N-cis-feruloyl 3-methyldopamine (cis-FMD) and N-trans-feruloyl 3-methyldopamine (trans-FMD) in Huai-niu-xi. The HPLC system uses an Inertsil ODS-P (5 µm, 250 mm times 4.6 mm I.D.) column with gradient elution, with acetonitrile and water as the mobile phase. A detector was set at 300 nm. Calibration graphs were constructed in the range 8.0-160.0 µg mL-1 for cis-FMD and 16.4-246.0 µg mL-1 for trans-FMD. Regression equations revealed good linear relationships (correlation coefficients: 0.9997-0.9998) between the peak-area to each constituent. The contents of two constituents of A. bidentata in six samples have been determined. This study using HPLC, also compared with those similar herbal medicines, Cyathula officinals (Amaranthaceae) and Strobilanthes forrestii (Acanthaceae), commonly used as an adulterant.
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JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES 27(4)727 ~736
