Analysis of Flavonoids and Coumarins in Ixeris laevigata var. oldhami By HPLC

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Title:	Analysis of Flavonoids and Coumarins in Ixeris laevigata var. oldhami By HPLC
Authors:	(Kuo-Liang Lu);(Kuo-Liang Lu);(Yoe-Ray Ku);溫國慶(Kuo-Ching Wen);(Li-Kang Ho);張永勳(Yuan Shiun Chang)*
Contributors:	藥學院中國藥學研究所;中國附醫藥劑部中藥局
Date:	2000
Issue Date:	2009-08-26 16:21:34 (UTC+8)
Abstract:	Tao-shang-tsao is the dried entire plant of Ixeris laevigata var. oldhami (Compositae) and a commonly used folk herb in Taiwan. To evaluate the quality of I. laevigata var. oldhami, a simple, rapid, and accurate high-performance liquid chrom-atographic (HPLC) method was developed for the assay of four flavonoids, apigenin, apigenin-7-O-glucoside, luteolin, and luteolin-7-O-glucoside, and two coumarins, esculetin and esculin. The present HPLC system uses an Inertsil ODS-2 column by gradient elution with acetonitrile and 0.1% (v/v) phosphoric acid as the mobile phase. Ethylparaben was used as an internal standard and detected at 254 nm. Regression equations revealed good linear relationships (correlation coefficients: 0.9998-0.9999) between the peak-area ratios of each constituent to ethylparaben. The relative standard deviations of these six constituents ranged between 0.56-3.04% (intra-day) and 0.79-4.39% (inter-day). The contents of six constituents of I. laevigata var. oldhami in 7 crude drugs have been determined. This study also compared their constituents with those of similar folk herbs, I. chinensis and Taraxacum formosanum, commonly used as an adulterant, by their HPLC chromatograms.
Relation:	JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES 23(16)2573~2583
Appears in Collections:	[Graduate Institute of Chinese Pharmaceutical Science] Journal articles

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