The extractions of ginsenosides Rg1 and Rb1 from ginseng root under atmospheric pressure by focused microwave-assisted technique have been investigated. The parameters used for the optimization were solvent composition, extraction time, and applied microwave power. The ginsenosides were quantified by high-performance liquid chromatography equipped with UV/Vis detector. The results of the 15-min microwave-assisted extraction (0.28% of Rg1 obtained in 70% water–ethanol and 1.31% of Rb1 obtained in 30% water–ethanol under 150 W of microwave power) were better than that from 10-h conventional solvent extraction (0.22% of Rg1 and 0.87% of Rb1 obtained in 70% water–ethanol).