Karyotypic evolution of a novel cervid satellite DNA family isolated by microdissection from the Indian muntjac Y-chromosome

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Title:	Karyotypic evolution of a novel cervid satellite DNA family isolated by microdissection from the Indian muntjac Y-chromosome
Authors:	李月君(Y.-C. Li);鄭雅銘(Y.-M. Cheng);謝麗嬌(L.-J. Hsieh);(O. A. Ryder);(F.Yang);廖淑如(S.-J. Liao);蕭光明(Kuang-Ming Hsiao);蔡輔仁(Fuu-Jen Tsai);蔡長海(Chang-Hai Tsai);林齊強(C. C. Lin)*
Contributors:	醫學院醫學研究所;中國附醫醫學研究部染色體實驗室
Date:	2005-04
Issue Date:	2009-08-26 16:16:42 (UTC+8)
Abstract:	A minilibrary was constructed from DOP-PCR products using microdissected Y-chromosomes of Indian muntjac as DNA templates. Two microclones designated as IM-Y4-52 and IM-Y5-7 were obtained from negative screening of all three cervid satellite DNAs (satellites I, II, and IV). These two microclones were 295 and 382 bp in size, respectively, and shared sim70% sequence homology. Southern blot analysis showed that the IM-Y4-52 clone was repetitive in nature with an sim0.32-kb register in HaeIII digest. Sequence comparison revealed no similarities to DNA sequences deposited in the GenBank database, suggesting that the microclone sequences were from a novel satellite DNA family designated as cervid satellite V. A subclone of an Indian muntjac BAC clone which screened positive for IM-Y4-52 had a 3,325-bp insert containing six intact monomers, four deleted monomers, and two partial monomers. The consensus sequence of the monomer was 328 bp in length and shared more than 80% sequence homology with every intact monomer. A zoo blot study using IM-Y4-52 as a probe showed that the strong hybridization with EcoRI digested male genomic DNA of Indian muntjac, Formosan muntjac, Chinese muntjac, sambar deer, and Chinese water deer. Female genomic DNA of Indian muntjac, Chinese water deer, and Formosan muntjac also showed positive hybridization patterns. Satellite V was found to specifically localize to the Y heterochromatin region of the muntjacs, sambar deer, and Chinese water deer and to chromosome 3 of Indian muntjac and the X-chromosome of Chinese water deer.
Relation:	CHROMOSOMA (114)28~38
Appears in Collections:	[Graduate Institute of Medical Science] Journal articles

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