Modulation of NO and cytokines in microglial cells by Cu/Zn-SOD

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Title:	Modulation of NO and cytokines in microglial cells by Cu/Zn-SOD
Authors:	張淑緘(Chang SC);高銘欽(Ming-Ching Kao);傅明慈(Ming-Tsze Fu);林棋財(Chi-Tsai Lin)*
Contributors:	醫學院臨床醫學研究所
Keywords:	Cu/Zn-SOD;Microglia;BV-2 cell;LPS;NO;IL-1β;TNF-α;Free radicals
Date:	2001-07
Issue Date:	2009-08-26 16:12:44 (UTC+8)
Abstract:	The activation of microglial cells in response to neuropathological stimuli is one of the prominent features of human neurodegenerative diseases. Cytokines such as IL-1β and TNF-α and inflammation-related enzymes such as inducible nitric oxide synthase are usually induced during the activation of microglial cells. We investigated the modulation of the activation of microglial cell by transfecting a Cu/Zn-SOD cDNA into BV-2 cells. Parental and transfected BV-2 cells were then subjected to LPS stimulation. The results showed that in Cu/Zn-SOD-transfected BV-2 cells, the expression and activity of Cu/Zn-SOD increased. On the other hand, upon activation by LPS, these cells produced less NO, IL-1β, and TNF-α than the parental microglial cells. This finding suggests that superoxide may be an early signal triggering the induction of cytokines and that the transfected Cu/Zn-SOD may provide a neuroprotective function via suppression of microglial activation. In addition, this approach may provide a rationale for the development of treatments for neurodegenerative diseases.
Relation:	FREE RADICAL BIOLOGY AND MEDICINE 31(9)1084~1089
Appears in Collections:	[Graduate Institute of Clinical Medical Science] Journal articles

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