E5 proteins of human papillomavirus types 11 and 16 transactivate the c-fos promoter through the NF1 binding element.

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Title:	E5 proteins of human papillomavirus types 11 and 16 transactivate the c-fos promoter through the NF1 binding element.
Authors:	(Show-Li Chen)*;(Ying-Kuang Lin);李龍緣(Long-Yuan Li);(Yeou-Ping Tsao);(Hsiang-Yun Lo);(Won-Bo Wang);(Tzung-Chieh Tsai)
Contributors:	醫學院癌症生物學研究所;中國附醫分子醫學中心癌症生物學
Date:	1996-12
Issue Date:	2009-08-26 16:11:19 (UTC+8)
Abstract:	Human papillomavirus type 11 (HPV-11) and HPV-16 contain an E5 gene that can induce c-fos gene expression in mouse fibroblasts. This study investigated the human c-fos promoter characteristics by mapping the c- fos promoter sequence with several deletion and point mutants that confer responsiveness to E5 of HPV-11 or HPV-16. The mutant studies show that NF1 binding sequences within the c-fos promoter were crucial for the induction of the c-fos gene by E5, and the gel shift assay study suggested that E5 of both HPV-11 and HPV-16 is associated, perhaps indirectly, with this NF1 element in the transactivation of the human c-fos promoter. Using an inducible system, we demonstrate that increased induction of the HPV-11 E5 gene in cells led to increased transactivation of the NF1 element. In addition, the transactivating activity of a series of HPV-11 E5 mutants on the NF1 element had a strong correlation with their respective transforming activities.
Relation:	JOURNAL OF VIROLOGY 12(70)8558~8563
Appears in Collections:	[Graduate Institute of Cancer Biology] Journal articles

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