Amplification and Overexpression of Prosaposin in Prostate Cancer

CMUR > College of Medicine > Graduate Institute of Cancer Biology > Journal articles > Item 310903500/7064

Please use this identifier to cite or link to this item: http://ir.cmu.edu.tw/ir/handle/310903500/7064

Title:	Amplification and Overexpression of Prosaposin in Prostate Cancer
Authors:	(Shahriar Koochekpour)*;(Yujun Zhang);(Rameen Beroukhim);è¬åç²;(Matthias D. Hofer);(Haiyen E. Zhau);(Masao Hiraiwa);(Daniel Y. Pattan);(Joy L.Ware);(Ronald B. Luftig);(Konrad Sandhoff);(Charles L. Sawyers);(Kenneth J. Pienta);(Mark A. Rubin);(Robert L. Vessella);(William R. Sellers);(Oliver Sartor)
Date:	2005-05
Issue Date:	2009-08-26 16:11:06 (UTC+8)
Abstract:	We identified prosaposin (PSAP) as a secreted protein expressed in androgen-independent (AI) prostate cancer cells by cloning/sequencing, after probing a PC-3 cDNA library expressed in the Î»TriplEx phagemid expression vector with a polyclonal rabbit antibody generated against pooled human seminal plasma. PSAP is a neurotrophic molecule; its deficiency or inactivation has proved to be lethal in man and mice, and in mice, it leads to abnormal development and atrophy of the prostate gland, despite normal testosterone levels. We used Southern hybridization, quantitative real-time polymerase chain reaction, and/or single nucleotide polymorphism (SNP) array analysis, and we now report the genomic amplification of PSAP in the metastatic AI prostate cancer cell lines, PC-3, DU-145, MDA-PCa 2b, M-12, and NCI-H660. In addition, by using SNP arrays and a set of 25 punch biopsy samples of human prostate cancer xenografts (LAPC3, LuCaP 23.1, 35, 49, 58, 73, 77, 81, 86.2, 92.1, 93, 96, 105, and 115), lymph nodes, and visceral-organ metastases, we detected amplification of the PSAP locus (10q22.1) in LuCaP 58 and 96 xenografts and two lymph node metastases. In addition, AI metastatic prostate cancer cell lines C4-2B and IA8-ARCaP over-expressed PSAP mRNA without evidence of genomic amplification. Taken together with prior data that demonstrated the growth-, migration-, and invasion-promoting activities, the activation of multiple signal transduction pathways, and the antiapoptotic effect of PSAP (or one of its active domains, saposin C) in prostate cancer cells, our current observation of PSAP amplification or overexpression in prostate cancer suggests, for the first time, a role for this molecule in the process of carcinogenesis or cancer progression in the prostate.
Relation:	GENES CHROMOSOMES & CANCER (44 )351 ~364
Appears in Collections:	[Graduate Institute of Cancer Biology] Journal articles

Files in This Item:

File	Description	Size	Format
Amplification and overexpression of prosaposin in prostate cancer.doc		358Kb	Microsoft Word	1463	View/Open
Amplification and overexpression of prosaposin in prostate cancer.pdf		254Kb	Adobe PDF	1204	View/Open

Loading...