中國醫藥大學機構典藏 China Medical University Repository, Taiwan:Item 310903500/7029
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    jsp.display-item.identifier=請使用永久網址來引用或連結此文件: http://ir.cmu.edu.tw/ir/handle/310903500/7029


    题名: Anion exchanger inhibitor DIDS induces human poorly-differentiated maligant hepatocellular carcinoma HA22T cell apoptosis
    作者: (Chung-Jung Liu);(Jin-Ming Hwang);(Trang-Tiau Wu);(Yi-Hsien Hsieh);(Cheng-Chung Wu);(Yih-Shou Hsieh);(Chang-Hai Tsai);(Hsi-Chin Wu);黃志揚*;(Jer-Yuh Liu)*
    贡献者: 醫學院基礎醫學研究所
    关键词: HA22T hepatocellular carcinoma cells;Anion exchanger 2;DIDS;Apoptosis;Proliferation;Anion transport activity
    日期: 2008-01
    上传时间: 2009-08-26 16:09:47 (UTC+8)
    摘要: Anion exchangers (AEs) of the Cl-/HCO3- exchanger family contribute to the regulation of intracellular acid-base balance. Recently, we found that anion exchanger 2 (AE2) was significantly expressed in human hepatocellular carcinoma (HCC) and in poorly-differentiated human HCC HA22T/VGH cells. In the present study, we further explored the pharmacological function of AE in four human HCC cell lines (SK-Hep-1, HA22T/VGH, HepG2, and Hep3B) following the treatment of 4,4’-diisothiocyanatostilbene-2,2′-disulfonic acid (DIDS), an AEs specific inhibitor. After administrations with 400–1000 μM of DIDS, cell proliferation was greatly inhibited in a dose-dependent manner from 47.5 to 65.0% in higher malignant HA22T/VGH cells, but not in other cell lines. The results of 4,6-diamidino-2-phenylindole (DAPI) staining, DNA fragmentation and flow cytometric analysis further revealed that cell apoptosis induced by DIDS was also observed in HA22T/VGH cells. Therefore, these findings suggested that AE may be involved, in part, in the proliferation and survival of HA22T cells and could be a new potential therapeutic target against specific human HCC.
    關聯: MOLECULAR AND CELLULAR BIOCHEMISTRY 308(1-2 )117 ~125
    显示于类别:[基礎醫學研究所] 期刊論文

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