P38 mitogen-activated protein kinase pathways are involved in the hypertrophy and apoptosis of cardiomyocytes induced by Porphyromonas gingivalis conditioned medium

中國醫藥大學機構典藏 China Medical University Repository, Taiwan > 醫學院 > 基礎醫學研究所 > 期刊論文 > Item 310903500/7026

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题名:	P38 mitogen-activated protein kinase pathways are involved in the hypertrophy and apoptosis of cardiomyocytes induced by Porphyromonas gingivalis conditioned medium
作者:	(Wu HC);(Yeh YL);(Kuo WW);(Huang SK);(Kuo WH);(Hsieh DJ);(Wu CL);(Tsai CH);李信達(Shin-Da Lee);黃志揚(Chih-Yang Huang)
贡献者:	醫學院基礎醫學研究所
关键词:	periodontitis;P. gingivalis;myocardial cell;apoptotic;p38 signal pathways
日期:	2008-03
上传时间:	2009-08-26 16:09:45 (UTC+8)
摘要:	The surrounding medium of periodontal pathogen Porphyromonas gingivalis (P. gingivalis) increased cardiomyocyte hypertrophy and apoptosis whereas Actinobaeillus actinomycetemcomitans and Prevotella intermedia had no effects. The purpose of this study is to clarify the role of p38 pathway in P. gingivalis conditioned medium-induced H9c2 myocardial cell hypertrophy and apoptosis. DNA fragmentation, cellular morphology, nuclear condensation, p38 protein products, and mitochondrial-dependent apoptotic related proteins in cultured H9c2 myocardial cell were measured by agarose gel electrophoresis, immunofluorescence, DAPI, and western blotting following P. gingivalis conditioned medium and/or pre-administration of SB203580 (p38 inhibitor). The p38 protein products and associated activities in H9c2 cells were both upregulated by P. gingivalis conditioned medium. P. gingivalis conditioned medium increased cellular sizes, DNA fragmentation, nuclear condensation, mitochondrial Bcl2-associated death promoter (Bad), cytosolic cytochrome c (cyt c), and the activated form of caspase-9 proteins in H9c2 cells. The increased cellular sizes, DNA fragmentation, nuclear condensation, Bad, cyt c, and caspase-9 activities of H9c2 cells treated with P. gingivalis conditioned medium were all significantly reduced after pre-administration of SB203580. Our findings suggest that the activity of p38 signal pathway may be initiated by P. gingivalis conditioned medium and further activate mitochondrial-dependent apoptotic pathways leading to cell death in cultured H9c2 myocardial cells. Copyright © 2007 John Wiley & Sons, Ltd.
關聯:	CELL BIOCHEMISTRY AND FUNCTION 26(2)246 ~255
显示于类别:	[基礎醫學研究所] 期刊論文

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